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Merck
CN

70239-M

His•Bind® Purification Kit

Synonym(s):

IMAC, Protein purification

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About This Item

NACRES:
NA.56
UNSPSC Code:
41106500

Key Documents

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manufacturer/tradename

Novagen®

storage condition

OK to freeze

Quality Level

400

General description

HIS-BIND® purification kit includes pre-tested buffers specifically designed for IDA-based HIS-BIND Resin, enabling convenient and rapid one-step purification of proteins by metal affinity chromatography. It uses the HIS TAG®/HIS-BIND® technology, that leverages the affinity between neighboring histidine in the HIS TAG sequence and immobilized metal ions (such as Ni2+ or Co2+).

Application

HIS-BIND® Purification Kit has been used for: the purification of N-terminal 6-histidine-tagged aldehyde dehydrogenase isozyme, ALDH1A1 under nondenaturing conditions by nickel-NTA affinity chromatography, the purification of virus binding protein specific for Poliovirus Type 1, the purification of recombinant oE7, huhsp70 and oE7/huhsp70 proteins, to study the effective antitumor efficacy of human hsp70 and HPV16 oE7 fusion protein vaccine, the purification of recombinant exFPR1, MTBHsp70 and MTBHsp70-exFPR1 fusion proteins to study their role in cervical cancer immunotherapy

Features and Benefits

  • Includes HIS-BIND® resin, HIS-BIND® buffer kit and chromatography columns.
  • Solutions are included for Ni2+ charging, binding, washing, and elution.
  • Columns are pre-packed with 1.25 ml bed volume of Ni2+-charged HIS-BIND resin.

Other Notes

•10 mlHis•Bind Resin

•1His•Bind Buffer Kit

•pkg/4Chromatography Columns
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

signalword

Danger

Hazard Classifications

Aquatic Chronic 2 - Carc. 1A Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1C - Skin Sens. 1 - STOT RE 1 - STOT RE 2 Inhalation

Storage Class

3 - Flammable liquids

flash_point_f

86.0 °F

flash_point_c

30 °C

wgk

WGK 3

target_organs

respiratory tract irritation, respiratory tract irritation,Respiratory Tract

Regulatory Information

危险化学品
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Certificates of Analysis (COA)

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Human hsp70 and HPV16 oE7 fusion protein vaccine induces an effective antitumor efficacy
Jinbao Zong, et al.
Oncology Reports, 30(1), 407-412 (2013)
Construction of a Cloning System for the Mass Production of a Virus-Binding Protein Specific for Poliovirus Type 1
Sano D and Omura T
Applied and Environmental Microbiology, 71(5) (2005)
MTBHsp70-exFPR1-pulsed Dendritic Cells Enhance the Immune Response against Cervical Cancer
Cao G, et al.
Journal of Cancer, 10(25), 6364?6373-6364?6373 (2019)
A bioluminescence assay for aldehyde dehydrogenase activity
Duellman SJ, et al.
Analytical Biochemistry, 434(2) (2013)

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