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Merck
CN

70751

BugBuster® Ni-NTA His•Bind® Purification Kit

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About This Item

NACRES:
NA.56
UNSPSC Code:
41106500
Shipped in:
wet ice
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manufacturer/tradename

Novagen®

storage condition

OK to freeze

shipped in

wet ice

General description

BugBuster®Ni-NTA HIS-BIND® Purification Kit is used for protein purification. Ni-NTA HIS-BIND® Resin is high-performance Ni2+-charged agarose used for rapid, one-step purification of proteins containing a polyhistidine tag sequence.

Application

BugBuster®Ni-NTA HIS-BIND® Purification Kit has been used for the purification of His tagged proteins such as trehalose-6-phosphate phosphatase (TPP) , Ras(WT) proteins and CsgA (major curlin subunit) proteins.

Other Notes

•2 × 100 mlBugBuster Protein Extraction Reagent

•10,000 UBenzonase Nuclease, purity >90%

•10 mlNi-NTA His•Bind Resin

•pkg/4Chromatography Columns
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Multiple Toxicity Values, refer to MSDS (O)


pictograms

Flame

Storage Class

3 - Flammable liquids

wgk

WGK 3

signalword

Warning

hcodes

Hazard Classifications

Flam. Liq. 3



Certificates of Analysis (COA)

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Articles

This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.

Related Content

E. coli transformation with poly-histidine tagged constructs represents a common vehicle for protein production. Initial screening of small-scale cultures is routinely performed to identify clones producing the highest amounts of protein with the desired form and function. As the demand for greater throughput at this level has increased, so has the need for process simplification and greater reproducibility. To meet this need, we have developed a condensed workflow that can be performed in a single device.

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Global Trade Item Number

SKUGTIN
369071-5G04061837008450
70751-304055977273168