71456
BugBuster® Master Mix
Synonym(s):
BugBuster® Protein Extraction Reagent with Benzonase® Nuclease and rLysozyme solution, Cell Lysis Master Mix
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About This Item
form
liquid
Quality Level
manufacturer/tradename
Novagen®
storage condition
OK to freeze
shipped in
wet ice
storage temp.
2-8°C
General description
Application
- to resuspend and lyse the cell pellets during protein purification
- to extract proteins for immunoblotting
- as a bacterial lysis buffer for the preparation of inclusion bodies
Biochem/physiol Actions
Features and Benefits
- Efficient lysis of Gram-positive and Gram-negative bacteria
- Maximum recovery of active, soluble protein
- Capable of cell wall perforation without denaturing soluble protein
- Useful for the preparation of high purity inclusion bodies
- Compatible with protease and phosphatase inhibitor cocktail
- Offers a convenient, fast, and cost-effective solution for releasing the expressed target protein.
- The Master Mix eliminates the need to dilute or perform separate addition steps.
Legal Information
Disclaimer
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens
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Traditionally, protein purification from E. coli consists of four distinct phases: harvest, bacterial cell lysis, lysate clarification and protein purification. Bacterial lysis typically requires several time-consuming, hands-on steps, such as freeze/thaw cycles and sonication. These harsh lysis techniques may negatively impact protein quality and contribute to sample-to-sample variability. To maintain protein activity and integrity, detergent-based lysis buffers are routinely used to avoid mechanical protein extraction methods. Regardless of the lysis method used, centrifugation is traditionally required to pellet unwanted cell debris and permit recovery of the clarified lysate. The final step, purification, is frequently performed using affinity media specific for expressed epitope tags. Agarose-based media have typically been used, either as a slurry in microcentrifuge tubes or packed into gravity-driven or spin columns. While easier to manipulate, columns are greatly affected by lysate consistency and carryover of cell debris, which can lead to clogging of the column frits.
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