Skip to Content
Merck
CN

Key Documents

View All Documentation

71456

BugBuster® Master Mix

Synonym(s):

BugBuster® Protein Extraction Reagent with Benzonase® Nuclease and rLysozyme solution, Cell Lysis Master Mix

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

NACRES:
NA.32
UNSPSC Code:
41106511
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

form

liquid

Quality Level

200

manufacturer/tradename

Novagen®

storage condition

OK to freeze

shipped in

wet ice

storage temp.

2-8°C

General description

The BugBuster® Master Mix enables efficient protein purification and other related applications. It replaces the need for mechanical methods like the French press or sonication. The two available package sizes contain sufficient reagents for extracting proteins from either 20 grams or 100 grams of cell paste.

Application

BugBuster® Master Mix has been used:
  • to resuspend and lyse the cell pellets during protein purification
  • to extract proteins for immunoblotting
  • as a bacterial lysis buffer for the preparation of inclusion bodies


    

Biochem/physiol Actions

BugBuster® Protein Extraction Reagent is specifically designed to gently disrupt the cell wall of E. coli, allowing the release of soluble proteins. This unique formulation utilizes a detergent mix that can effectively perforate the cell wall without denaturing the soluble proteins. By combining BugBuster Reagent, rLysozyme solution, and Benzonase Nuclease, our BugBuster Master Mix enhances protein extraction efficiency and simplifies downstream processing of protein extracts.

Features and Benefits

  • Efficient lysis of Gram-positive and Gram-negative bacteria
  • Maximum recovery of active, soluble protein
  • Capable of cell wall perforation without denaturing soluble protein
  • Useful for the preparation of high purity inclusion bodies
  • Compatible with protease and phosphatase inhibitor cocktail

  • Offers a convenient, fast, and cost-effective solution for releasing the expressed target protein.
  • The Master Mix eliminates the need to dilute or perform separate addition steps.

Legal Information

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

12 - Non Combustible Liquids

Regulatory Information

常规特殊物品

This item has

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

Related Content

How to Automate HIS TAG® Protein Purification on the AAW™ Workstation

Read an automated protocol for protein purification using PureProteome™ nickel magnetic beads on the AAW™ automated assay workstation and see results comparing manual vs automated runs.

Protein Research Guide

Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.

蛋白提取_细胞裂解_蛋白质生物学-默克生命科学

细胞裂解和蛋白质溶解是进行有效分析和高效处理的关键步骤，默克分享提取方法包括酶法、化学法、机械法或几种方法的联合使用。

View All Related Content
Yong Y Peng et al.
Bioengineered, 5(6), 378-385 (2014-12-09)
The collagen like domain Scl2 from Streptococcus pyogenes has been proposed as a potential biomedical material. It is non-cytotoxic and non-immunogenic and can be prepared in good yield in fermentation. The Scl2 collagen domain is about a quarter of the
RT-QuIC assays for prion disease detection and diagnostics
Orru CD, et al.
Journal of biological and chemical chronicles, 185-203 (2017)
Daniel G Isom et al.
Proteins, 79(4), 1034-1047 (2011-03-10)
Protein thermodynamic stability is a fundamental physical characteristic that determines biological function. Furthermore, alteration of thermodynamic stability by macromolecular interactions or biochemical modifications is a powerful tool for assessing the relationship between protein structure, stability, and biological function. High-throughput approaches
View All Related Papers

Global Trade Item Number

SKUGTIN
71456-307790788053260
71456-404055977270150