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Millipore

BugBuster® Master Mix

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Synonym(s):
BugBuster® Protein Extraction Reagent with Benzonase® Nuclease and rLysozyme solution, Cell Lysis Master Mix

form

liquid

Quality Level

200

manufacturer/tradename

Novagen®

storage condition

OK to freeze

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

The BugBuster® Master Mix enables efficient protein purification and other related applications. It replaces the need for mechanical methods like the French press or sonication. The two available package sizes contain sufficient reagents for extracting proteins from either 20 grams or 100 grams of cell paste.

Application

BugBuster® Master Mix has been used:
  • to resuspend and lyse the cell pellets during protein purification
  • to extract proteins for immunoblotting
  • as a bacterial lysis buffer for the preparation of inclusion bodies


    

Biochem/physiol Actions

BugBuster® Protein Extraction Reagent is specifically designed to gently disrupt the cell wall of E. coli, allowing the release of soluble proteins. This unique formulation utilizes a detergent mix that can effectively perforate the cell wall without denaturing the soluble proteins. By combining BugBuster Reagent, rLysozyme solution, and Benzonase Nuclease, our BugBuster Master Mix enhances protein extraction efficiency and simplifies downstream processing of protein extracts.

Features and Benefits

  • Efficient lysis of Gram-positive and Gram-negative bacteria
  • Maximum recovery of active, soluble protein
  • Capable of cell wall perforation without denaturing soluble protein
  • Useful for the preparation of high purity inclusion bodies
  • Compatible with protease and phosphatase inhibitor cocktail

  • Offers a convenient, fast, and cost-effective solution for releasing the expressed target protein.
  • The Master Mix eliminates the need to dilute or perform separate addition steps.

Warning

Toxicity: Standard Handling (A)

Legal Information

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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FMRP inhibits translation elongation independent of mRNA G-quadruplexes
Scarpitti MR, et al.
bioRxiv : the preprint server for biology null
Development of a synthetic biosensor for chemical exchange MRI utilizing in silico optimized peptides
Fillion AJ, et al.
Nmr in Biomedicine, 36, e5007-e5007 (2023)
Matthew Thomas Doyle et al.
Molecular cell, 81(9), 2000-2012 (2021-03-12)
The β-barrel assembly machine (BAM) integrates β-barrel proteins into the outer membrane (OM) of Gram-negative bacteria. An essential BAM subunit (BamA) catalyzes integration by promoting the formation of a hybrid-barrel intermediate state between its own β-barrel domain and that of
Brian D Huang et al.
Nature communications, 13(1), 3901-3901 (2022-07-07)
Bacteroides species are prominent members of the human gut microbiota. The prevalence and stability of Bacteroides in humans make them ideal candidates to engineer as programmable living therapeutics. Here we report a biotic decision-making technology in a community of Bacteroides
Daniel G Isom et al.
Proteins, 79(4), 1034-1047 (2011-03-10)
Protein thermodynamic stability is a fundamental physical characteristic that determines biological function. Furthermore, alteration of thermodynamic stability by macromolecular interactions or biochemical modifications is a powerful tool for assessing the relationship between protein structure, stability, and biological function. High-throughput approaches
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Articles

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

Related Content

Lysis & Protein Extraction

An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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