Amino PEGA resin

PEGA resins consist of dimethyl acrylamide and mono-2-acrylamidoprop-1-yl^{[2-aminoprop-1-yl]} polyethylene glycol cross-linked with bis 2-acrylamidoprop-1-yl polyethyleneglycol. These supports swell extensively in a wide range of solvents and are permeable to macromolecules up to 35 kD, making them ideal for the preparation of combinatorial libraries, affinity purification and on-resin enzyme assays. For applications of these resins in SPPS and SPOS see ^{[1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18]}.Note: PEGA resins are supplied swollen in ethanol, with 1g dry resin corresponding to approximately 15 mL of swollen resin. As the beads of PEGA resins become very sticky and easily damaged when shrunk or dried, they are best handled in a swollen state. To use, the appropriate amount of swollen resin should be weighed into a reaction vessel and any residual ethanol removed by copious washings with the appropriate solvent or solvent mixture. After peptide assembly, the resin should be washed with DCM and transferred to the cleavage vessel. Excess DCM can be removed under vacuum, and the shrunk resin treated with the TFA cocktail. The characteristics of the swollen resin, its robustness and high permeability enables its use in both batch and continuous flow synthesis apparatus.

Associated Protocols and Technical Articles
Protocols for Loading of Peptide Synthesis Resins Literature references

Literature references

^[1] M. Meldal, et al. (1992) Tetrahedron Lett., 33, 3077.
^[2] F. I. Auzanneau, et al. (1994) J. Peptide Sci., 1, 31.
^[3] M. Meldal in ′Peptides 1992, Proc. 22nd European Peptide Symposium′, C. H. Schneider & A. N. Eberle (Eds), ESCOM, Leiden, 1993, pp.61.
^[4] M. Meldal, et al. in ′Innovation & Perspectives in Solid Phase Synthesis, 3rd International Symposium′, R. Epton (Eds), Mayflower Worldwide, Birmingham, 1994, pp. 259.
^[5] M. Meldal, et al. (1993) Int. J. Peptide Protein Res., 41, 250.
^[6] M. Meldal, et al. (1994) J. Chem. Soc., Chem. Commun., 849.
^[7] M. Meldal, et al. (1994) Proc. Natl. Acad. Sci. USA, 91, 3314.
^[8] M. Renil, et al. (1998) J. Peptide Sci., 4, 195.
^[9] J. C. Spetzler, et al. (1998) J. Peptide Sci., 4,128.
^[10] M. Meldal, et al. (1998) J. Peptide Sci., 4 ,83.
^[11] J. A. Camarero, et al. (1998) J. Peptide Res., 51, 303.
^[12] J. A. Camarero, et al. (2000) Lett. Pept. Sci., 7,17.
^[13] O. Melnyk, et al. (2001) J. Org. Chem., 66, 4153.
^[14] J. Buchardt, et al. (2000) J. Comb. Chem., 2, 624.
^[15] J. F. Tolberg, et al. (2002) J. Org. Chem., 67, 4143.
^[16] J. Bondebjerg, et al. (2002) J. Am. Chem. Soc., 124, 11046.
^[17] J. Tulla-Puche, et al. (2004) J. Org. Chem., 69, 4101.
^[18] S. Aggarwal, et al. (2005) Biomaterials, 26, 6077.

Replaces: 01-64-0100

Color (visual): white to yellow to beige
Appearance of substance (visual): beads
Loading (determined from the substitution of the Fmoc-Leu loaded resin): 0.30 - 0.50 mmol/g
Swelling Volume (in DMF): lot specific result
Swelling Volume (in water): lot specific result
dry resin %: lot specific result
The resin is sold swollen in ethanol. 50 - 100 mesh

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany