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AB144P

Sigma-Aldrich

Anti-Choline Acetyltransferase Antibody

Chemicon®, from goat

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Synonym(s):
Choline acetylase, acetyl CoA:choline O-acetyltransferase, choline acetyltransferase, ChAT
eCl@ss:
32160702
NACRES:
NA.41

biological source

goat

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, opossum, guinea pig, rat, mouse, zebrafish (THE JOURNAL OF COMPARATIVE NEUROLOGY 474:75–107 (2004).), avian, monkey, chicken

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

NCBI accession no.

NM_020549.3

UniProt accession no.

P28329

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

chicken ... Chat(395314)
human ... CHAT(1103)
mouse ... Chat(12647)
rat ... Chat(290567)
rhesus monkey ... Chat(709977)

General description

Choline acetyltransferase (ChAT) has a molecular weight of about 68 kDa. It is synthesized in the parakaryon and is transported to the axon terminals. ChAT belongs to the choline/carnitine acyltransferase family. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. Choline acetyltransferase is found in cholinergic neurons in brain and spinal cord.

Specificity

Expected to cross-react with Avian, chicken, guinea pig, monkey, rat, and opposum.

Immunogen

Human placental enzyme.

Application

Anti-Choline Acetyltransferase Antibody has been used in:
  • immunohistochemical staining
  • western blotting
  • immunocytochemistry

Immunohistochemistry:
A previous lot of this antibody was used at 1:100.

Optimal working dilutions and protocols must be determined by end user.

Biochem/physiol Actions

Choline acetyltransferase (CHAT) is involved in the synthesis of acetylcholine in neurons. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CHAT catalyzes the transfer of an acetyl group between acetyl-coenzyme A and choline. Mutations in the gene encoding this protein have been associated with congenital neuromuscular disorders.

Quality

Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected CHAT on 10 μg of mouse brain lysates.

Target description

70/74 kDa

Physical form

Affinity purified
Purified goat polyclonal in buffer containing 5 mg/mL BSA and 0.2% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Avoid repeated freeze/thaw cycles.
For short term storage (3-4 weeks) maintain at 2-8°C.

Analysis Note

Control
Human placenta lysates, rat forebrain/rostral hypothalamus.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Research Category:
Neuroscience

Research Sub Category:
Neurotransmitters & Receptors

Neuronal & Glial Markers

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

含少量动物源组分生物产品
常规特殊物品

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Choline acetyltransferase structure reveals distribution of mutations that cause motor disorders
Cai Y, et al.
The Embo Journal, 2047-2047 (2004)
S D Motts et al.
Neuroscience, 160(1), 103-114 (2009-03-14)
We combined retrograde tracing with immunohistochemistry for choline acetyltransferase to identify the source of cholinergic input to the inferior colliculus (IC) in guinea pigs. Injection of a retrograde tracer into one IC labeled cells in many brainstem nuclei. Retrogradely-labeled cells
Javier Bernácer et al.
PloS one, 2(11), e1174-e1174 (2007-12-15)
The striatum (caudate nucleus, CN, and putamen, Put) is a group of subcortical nuclei involved in planning and executing voluntary movements as well as in cognitive processes. Its neuronal composition includes projection neurons, which connect the striatum with other structures
Blood pressure regulation by CD4+lymphocytes expressing choline acetyltransferase
Olofsson PS, et al.
Nature Biotechnology, 34(10), 1066-1071 (2016)
Uniformity detector retinal ganglion cells fire complex spikes and receive only light-evoked inhibition.
Sivyer, B; Taylor, WR; Vaney, DI
Proceedings of the National Academy of Sciences of the USA null
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