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AB1504

Sigma-Aldrich

Anti-Glutamate receptor 1 Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
AMPA 1, AMPA-selective glutamate receptor 1, Glutamate receptor ionotropic, AMPA 1, glutamate receptor, ionotropic, AMPA 1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, horse, mouse, human

species reactivity (predicted by homology)

platypus (based on 100% sequence homology), equine (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NP_000818.2

UniProt accession no.

P42261

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GRIA1(2890)

General description

Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. GluR1 is believed to mediate calcium influx, and has a vital function in synaptic plasticity related to learning. Reduction in GluR1 has been caused by FMR1 gene mutation and contributes to fragile X syndrome. Changes in expression of GluR1 receptors on dopamine neurons have been correlated with sensitization of drugs of abuse.

Specificity

This antibody recognizes Glutamate receptor 1 at the cytoplasmic domain.

Immunogen

Epitope: Cytoplasmic domain
KLH-conjugated linear peptide corresponding to human Glutamate receptor 1 at the cytoplasmic domain.

Application

Immunohistochemistry Analysis: 1:300 dilution of this antibody has been shown to detect Glutamate receptor 1 (GluR1) in rat brain tissue.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
This Anti-Glutamate receptor 1 Antibody is validated for use in WB, IH(P), IP for the detection of Glutamate receptor 1.

Quality

Evaluated by Western Blot in mouse brain lysate.

Western Blot Analysis: 1:1000 dilution of this antibody detected Glutamate receptor 1 (GluR1) on 10 µg of mouse brain lysate.

Target description

Observed at ~106 kDa

Physical form

Affinity purified
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide and 5mg/ml BSA.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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SNAP-25 is a target of protein kinase C phosphorylation critical to NMDA receptor trafficking.
Lau, CG; Takayasu, Y; Rodenas-Ruano, A; Paternain, AV; Lerma, J; Bennett, MV; Zukin, RS
The Journal of Neuroscience null
Brain-derived neurotrophic factor activation of CaM-kinase kinase via transient receptor potential canonical channels induces the translation and synaptic incorporation of GluA1-containing calcium-permeable AMPA receptors.
Fortin, DA; Srivastava, T; Dwarakanath, D; Pierre, P; Nygaard, S; Derkach, VA; Soderling, TR
The Journal of Neuroscience null
E Polgár et al.
Neuroscience, 167(4), 1192-1204 (2010-03-23)
Lamina I of the spinal cord contains many projection neurons that express the neurokinin 1 receptor (NK1r). It has been reported that these cells can undergo long-term potentiation (LTP), which may result from insertion of AMPA-type glutamate receptors (AMPArs) containing
Involvement of AMPA receptors in the antidepressant-like effects of lithium in the mouse tail suspension test and forced swim test.
Gould, TD; O'Donnell, KC; Dow, ER; Du, J; Chen, G; Manji, HK
Neuropharmacology null
Ping He et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 26(1), 334-345 (2011-10-11)
The distribution of postsynaptic glutamate receptors has been shown to be regulated by proimmunocytokine tumor necrosis factor α (TNF-α) signaling. The role of TNF-α receptor subtypes in mediating glutamate receptor expression, trafficking, and function still remains unclear. Here, we report
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