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Merck
CN

AB1526P

Anti-Nerve Growth Factor-β Antibody, pain

Chemicon®, from rabbit

Synonym(s):

NGFbeta

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA, IHC, WB
Citations:
9

Key Documents

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biological source

rabbit

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

chicken, human, mouse, rat

should not react with

bovine

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

NCBI accession no.

NM_002506.2

UniProt accession no.

P01138

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... NGF(4803)

Immunogen

Mouse nerve growth factor-beta purified from salivary glands.

Application

Immunohistochemistry: 1-2 μg/mL (see suggested protocol).

Immunoblotting: 1-2 μg/mL

Bioassays (Inhibition of biological activity in vitro): 1-2 μg/mL

in vivo animal studies: use neat

ELISA: 1-2 μg/mL

Optimal working dilutions must be determined by the end user.

BIOLOGICAL ACTIVITY:

This antibody completely inhibits neuron survival and the outgrowth actions of mouse NGF in chicken dorsal root ganglion in vitro.
Research Category
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
This Anti-Nerve Growth Factor-β Antibody, pain is validated for use in ELISA, WB, IH, FUNC for the detection of Nerve Growth Factor-β.

Biochem/physiol Actions

Nerve growth factor-beta (NGF). Less than 1% cross-reactivity against recombinant human BDNF, NT3 and NT4 by ELISA.

Physical form

Affinity purified immunoglobulin. Lyophilized from PBS. Contains no preservative. Reconstitute to desired volume with sterile distilled water. Suggested concentration 0.5-1.0 mg/mL.

Preparation Note

Maintain at lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20 to -70°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.

Other Notes

Replaces: 04-1119

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Immunohistochemical distribution of NGF, BDNF, NT-3, and NT-4 in adult rhesus monkey brains.
Zhang, HT; Li, LY; Zou, XL; Song, XB; Hu, YL; Feng, ZT; Wang, TT
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society null

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