Anti-Nerve Growth Factor Receptor Antibody, p75

Nerve Growth Factor Receptor p75 (UniProt: Q9Z0W1; also known as Tumor necrosis factor receptor superfamily member 16, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, NGF receptor, CD271) is encoded by the Ngfr (also known as Tnfrsf16) gene in murine species. Nerve Growth Factor Receptor p75 is a single-pass type I, homodimeric membrane protein that can serve as a low affinity receptor for NGF, BDNF, NT-3, and NT-4. It is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off in the mature form. It has an extracellular domain (aa 22-246), a transmembrane domain (aa 247-265), and a cytoplasmic domain (aa 266-417). Its expression is shown to oscillate in a circadian manner in the suprachiasmatic nucleus (SCN) of the brain and in liver and expression is seen at higher levels during the light period and lower during dark period. Depending on the developmental stage, cell type, and whether they are unbound or ligand bound, and the type of ligand, p75 can activate apoptosis or survival. During development, it signals toward apoptosis to eliminate unnecessary neurons. Its death domain is localized to amino acids 346-411, which is responsible for its interaction with RAN-binding protein 9 (RANBP9). Neurotrophin binding to p75 can activate the MAP kinase pathway leading to cell survival or apoptosis depending on the cell type or ligand type.

It has been reported that this antibody does not recognize rat p75 by Western blot. See MAB365 for rat reactive antibody using Western blotting.

p75 nerve growth factor receptor (p75 Neurotrophin receptor, low affinity neurotrophin receptor), extracellular domain.

Epitope: extracellular

Recombinant mouse NGF Receptor p75 extracellular fragment.

Anti-Nerve Growth Factor Receptor Antibody, p75 is an antibody against Nerve Growth Factor Receptor for use in IC, IH(P),WB and IP.

Immunoprecipitation:
1:500 - 1:5000 dilution of this antibody immunoprecipitated NGF Receptor p75 from 10 µg of PC12 cell lysate.

Immunohistochemistry(paraffin):
1:50 and 1:300 dilution from a previous lot detected NGF Receptor p75 using IHC-Select Detection with HRP-DAB.

Immunocytochemistry:
1:500 dilution from a previous lot detected NGF Receptor p75 in rat PC12 cells.

Research Category
Neuroscience

Research Sub Category
Neurochemistry & Neurotrophins

Routinely evaluated by immunoprecipitation on PC12 cell lysates.

Immunoprecipitation (IP): 1:500 - 1:5000 dilution of this antibody immunoprecipitated NGF Receptor p75 from 10 µg of PC12 cell lysate.

44.7 kDa

Replaces: 04-1111

Rabbit polyclonal serum containing 0.05% NaN3.

Unpurified

Stable for 1 year at -20ºC from date of receipt.

Control
Rat PC12 cells.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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