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AB1559

Sigma-Aldrich

Anti-Adenosine A2a Receptor Antibody

serum, Chemicon®

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eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

pig, bovine, rat, human, canine

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

suitability

not suitable for immunohistochemistry

NCBI accession no.

NM_000675.4

UniProt accession no.

P29274

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ADORA2A(135)

Immunogen

Synthetic 30 amino acid peptide corresponding to amino acids 383-412 of the canine adenosine A2a receptor near the C-terminal intracellular domain (30 amino acids) of the cloned canine A2a receptor. The A2a sequence selected in not present in A1, A2b and A3 receptors.

Application

This Anti-Adenosine A2a Receptor Antibody is validated for use in ELISA, WB for the detection of Adenosine A2a Receptor.
Western blot: 1:1,000-1:5,000 (Chemiluminescence technique), 40-100 μg membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.

ELISA: 1:10,000-1:50,000 (50-100 ng A2aR peptide (Cat. Number AG285)/well)

Optimal working dilutions must be determined by end user.

Physical form

Rabbit antiserum. Liquid containing 0.1% sodium azide.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

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Kah Y Tan-Allen et al.
Experimental eye research, 80(5), 687-696 (2005-05-03)
Previous studies indicated that adenosine can increase [cAMP](i) and stimulate fluid transport by corneal endothelium. The purpose of this study was to determine which adenosine receptor subtype(s) are expressed and to examine their functional roles in modulating [cAMP](i), [Ca(2+)](i) and
Patrycja Bolewska et al.
Neuroscience journal, 2019, 5950818-5950818 (2019-07-06)
There is an excellent correlation between the age when alcohol consumption begins and the likelihood of lifelong problems with alcohol abuse. Alcohol use often begins in adolescence, a time marked by brain development and maturation of numerous brain systems. Rats
Hendrik Busse et al.
Cardiovascular drugs and therapy, 30(6), 551-558 (2016-10-21)
Mechanical and morphological ischemia and reperfusion (I/R) injury is reduced in septic hearts. The mechanism behind this "cardioprotection" is less well understood. As adenosine receptors play a major role for cardioprotection in non-septic hearts, we investigated the influence of adenosine
Dasiel O Borroto-Escuela et al.
Frontiers in pharmacology, 9, 829-829 (2018-09-15)
The A2A adenosine (A2AR) and D2 dopamine (D2R) receptors form oligomers in the cell membrane and allosteric interactions across the A2AR-D2R heteromer represent a target for development of drugs against central nervous system disorders. However, understanding of the molecular determinants
Zhiwei Shi et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 31(37), 13118-13127 (2011-09-16)
Although retinal bipolar cells represent a morphologically well defined population of retinal interneurons, very little is known about the developmental mechanisms that regulate their processing. Furthermore, the identity of specific bipolar cell types that function in distinct visual circuits remains

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