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Merck
CN

AB1586

Anti-Ubiquitin Antibody

serum, Chemicon®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-Ubiquitin Antibody, serum, Chemicon®

biological source

sheep

conjugate

unconjugated

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, sheep, eukaryotes, rabbit

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NM_021009.4

UniProt accession no.

P62988

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... UBAP2(55833)

Preparation Note

Maintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

Detect Ubiquitin using this Anti-Ubiquitin Antibody validated for use in WB, IH.
Immunohistochemistry: 1:2,000-1:5,000.

Immunoblotting: 1:1,000-1:2,000 (depending on detection system used).

Optimal working dilutions must be determined by end user.
Research Category
Protein Trafficking
Research Sub Category
Ubiquitin & Ubiquitin Metabolism

Biochem/physiol Actions

Recognizes both free ubiquitin and ubiquitin-protein conjugates on Western blots. Labels tangles and Lewy bodies in paraffin or frozen human brain sections.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Ubiquitin from bovine red blood cells coupled to chicken gamma globulin.

Other Notes

Replaces: 04-454

Physical form

Sheep serum. Lyophilized, no preservatives. Reconstitute with 100 mL of sterile distilled water. Centrifuge to remove any residue.

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Xiao-Wen Lin et al.
PloS one, 8(6), e67633-e67633 (2013-06-27)
Toll-like receptors (TLRs) play a pivotal role in the defense against invading pathogens by detecting pathogen-associated molecular patterns (PAMPs). TLR4 recognizes lipopolysaccharides (LPS) in the cell walls of Gram-negative bacteria, resulting in the induction and secretion of proinflammatory cytokines such

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