Anti-Adenosine A1 Receptor Antibody

Recognizes Adenosine A1 receptor (A1R). Recognizes the 79 kDa and 39 kDa bands of in cortical membranes from rat, porcine and lamb. The 79 kDa band can be converted to 39 kDa band by agonist or antagonist treatment and could represent a A1R dimers.

Amino acid sequence corresponding to the 3rd extracellular domain (amino acids 163-176, GEPVIKCEFEKVIS) of the rat A1R gene.

Anti-Adenosine A1 Receptor Antibody detects level of Adenosine A1 Receptor & has been published & validated for use in ELISA, IC, IH & WB.

Immunohistochemistry: 10-50 μg/mL on frozen tissue sections. Light fixation is recommended. 2% 0.2% picric acid {Cooke, HJ (1999) AJP Gastrointest Liver Physiol 276:4516-4626}

Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity.

Western blot: 1-10 μg/mL (Chemiluminescence technique): Membrane preparations are recommended for enhanced signals {Smith et al (2001) Biochem Pharmacology 61:237-244} Higher concentrations of the antibody may be necessary if calorimetric detection or milk-based antibody diluent are used.

ELISA: 1:10,000-1:50,000 (50-100 ng A1R peptide (Cat. Number AG289)/well)

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Circadian Rhythm & Sleep

79 kDa and 39 kDa

Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.

ImmunoAffinity Purified

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Brain tissue

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.