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AB1590P

Sigma-Aldrich

Anti-Adenosine A3 Receptor Antibody, extracellular (a.a. 154-168)

Chemicon®, from rabbit

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eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

NM_000677.3
NM_001081976.1
NM_020683.6

UniProt accession no.

P33765

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ADORA3(140)

Specificity

Specific for Adenosine A3 receptor. Based on mRNA studies, the A3R has been found in rat testis and lung, kidneys, heart and brain. Other studies have found the mRNA to be highest in lung, spleen and pineal glands and moderate levels in brain kidney and testis.

Immunogen

Amino acid sequence corresponding to the 3rd extracellular domain (15 amino acids) of the rat A3R gene.
Epitope: extracellular (a.a. 154-168)

Application

Anti-Adenosine A3 Receptor Antibody, extracellular (a.a. 154-168) is an antibody against Adenosine A3 Receptor for use in ELISA, IC & WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Circadian Rhythm & Sleep
Western blot: 1-10 micrograms per mL: A3 receptors are different sizes in different species & tissues; A3R from rat brain membranes runs as a 40kDa band, 52kDa in rabbit uroepithelium membranes (Yu, W et al. Am J. Physiol Cell Physiol 2006 in press); use proteinase inhibitors and membrane preparations for best signals.

ELISA: 1:10,000-1:50,000 (50-100 ng A3R peptide (Catalog Number AG294)/well)

Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity.

Optimal working dilutions must be determined by end user.

Physical form

Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Neus Garcia et al.
Journal of anatomy, 225(1), 109-117 (2014-04-24)
To date, four subtypes of adenosine receptors have been cloned (A(1)R, A(2A)R, A(2B)R, and A(3)R). In a previous study we used confocal immunocytochemistry to identify A(1)R and A(2A)R receptors at mouse neuromuscular junctions (NMJs). The data shows that these receptors
Joana Ferreira-Silva et al.
International journal of molecular sciences, 21(19) (2020-10-04)
Glaucoma is a progressive chronic retinal degenerative disease and a leading cause of global irreversible blindness, characterized by optic nerve damage and retinal ganglion cell (RGC) death. Elevated intraocular pressure (IOP) is a main risk factor of glaucoma. Neuroinflammation plays

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