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Merck
CN

AB19012

Anti-Anti-Laminin (LAMA1) antibody

CHEMICON®, rabbit polyclonal

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
human
Application:
ELISA, IHC, IP, WB
Citations:
34
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Product Name

Anti-Laminin Antibody, Chemicon®, from rabbit

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Immunogen

Full length human laminin. Immunization occurred after single-band purity was assessed by SDS-PAGE.

Application

Detect Laminin using this Anti-Laminin Antibody validated for use in ELISA, IP, WB, IH.
Immunoblot: 1:500-1:2000

ELISA: 1:4000-1:8000

Immunohistochemistry: 1:50-1:200

Immunoprecipitation: Condition must be determined by the end user

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins collagen and non-collagen extracellular matrix proteins to remove unwanted reactivities. Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with most mammalian laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI collagens or fibronectin. Non-specific cross reaction of anti-laminin antibodies with other human serum proteins or non-laminin extracellular matrix proteins is negligible.

Physical form

Affinity purified immunoglobulin. Liquid at 1 mg/mL in 0.125 M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0 with 0.01% Sodium Azide.
Format: Purified

Preparation Note

The undiluted antibody solution is stable for approximately 6 months stored between 2 and 8° C. For extended storage mix with an equal volume of glycerol, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany


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pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Repr. 1B

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Notch3 null mutation in mice causes muscle hyperplasia by repetitive muscle regeneration.
Takeo Kitamoto,Kazunori Hanaoka
Stem Cells null
Cathepsin-L influences the expression of extracellular matrix in lymphoid organs and plays a role in the regulation of thymic output and of peripheral T cell number.
Gabriela Lombardi, Dalia Burzyn, Juliana Mundi?ano, Paula Berguer, Pedro Bekinschtein et al.
Journal of immunology (Baltimore, Md. : 1950) (1950)
Extracellular matrix-mediated membrane-type 1 matrix metalloproteinase expression in pancreatic ductal cells is regulated by transforming growth factor-beta1.
Ottaviano, AJ; Sun, L; Ananthanarayanan, V; Munshi, HG
Cancer Research null



Global Trade Item Number

SKUGTIN
AB1901204053252626876