Anti-MMP-2 Antibody, catalytic domain

E. coli-expressed active rat 72 kDa type IV collagenase (catalytic domain)

Epitope: catalytic domain

Detect MMP-2 using this Anti-MMP-2 Antibody, catalytic domain validated for use in IP & WB.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

Western blot: 1:2,000

Immunoprecipitation: 1:500

MMP-2 [Gelatinase-A] is constitutively produced in quiescent cells and tissues, and the enzyme has a high specific activity against denatured collagen. The low protein levels produced in cell culture (pg/mL) are often below the threshold of detection by standard Western Blotting. The enzyme can be concentrated from culture media by gelatin-agarose affinity chromatography (Goldberg, 1992) or immunoprecipitation. This can prevent appearance of a spurious band of antibody binding in the vicinity of 68 kDa displayed by some concentrated media.

Optimal working dilutions must be determined by end user.

Recognizes rat MMP-2. Exhibits no cross-reactivity with other MMP family members. The antibody was generated using E. coli-expressed active rat 72 kDa type IV collagenase (catalytic domain) as an immunogen.

Purified immunoglobulin. Liquid in PBS, containing 0.05% sodium azide.

Format: Purified

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-1048

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