Recombinant mouse adiponectin from E. coli.

Anti-Adiponectin Antibody detects level of Adiponectin & has been published & validated for use in ELISA & WB.

Research Category
Metabolism

Research Sub Category
Lipid Metabolism & Weight Regulation

Western blot: 1:5,000-1:10,000 using ECL. Reacts with the ~35 kDa adiponectin protein. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature.

ELISA, direct and indirect: 1:5,000-1:10,000.

Optimal working dilutions must be determined by the end user.

Recognizes mouse adiponectin. Recognizes both natural and recombinant protein.

SPECIES REACTIVITIES:

There is weak reactivity with rat. Reactivity with other species has not been confirmed.

Format: Purified

Purified immunoglobulin in PBS, pH 7.4.

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: MABS176

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.