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Merck
CN

AB3517

Anti-Polyhistidine Tag Antibody

Chemicon®, from chicken

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43
eCl@ss:
32160702
Clone:
polyclonal
Technique(s):
ELISA: suitable, immunocytochemistry: suitable, western blot: suitable
Application:
ELISA, ICC, WB
Citations:
1

Key Documents

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biological source

chicken

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunocytochemistry: suitable, western blot: suitable

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

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Immunogen

Synthetic peptide consisting of six histidines.

Application

Anti-Polyhistidine Tag Antibody detects level of Polyhistidine Tag & has been published & validated for use in ELISA, WB & IC.
Immunoblotting: 1:250-1:2,000

Immunocytochemistry: 1:250-1:1,000

ELISA: 1:250-1:2,000

Optimal working dilutions must be determined by the end user.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Biochem/physiol Actions

Polyhistidine tag, 6-His Tag containing proteins

Physical form

Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Rebba C Boswell-Casteel et al.
Molecules (Basel, Switzerland), 23(4) (2018-03-23)
Equilibrative nucleoside transporters (ENTs) are polytopic membrane transporters responsible for the translocation of nucleosides, nucleobases-to a lesser extent-and nucleoside analog therapeutics across cellular membranes. ENTs function in a diffusion controlled bidirectional manner and are thought to utilize an alternating access

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