Anti-Na+/K+/Cl- Cotransporter 2 Antibody

A 15 amino acid peptide sequence near the C-terminus of rat NKCC2 (Haas et al. 1987; Gamba et al. 1994; Simon et al. 1996; Mount et al. 1995; Igarashi et al. 1995; Mount et al. 1998). The immunogen peptide is available (catalog number AG205) for use in pre-absortion controls.

Anti-Na+/K+/Cl-Cotransporter 2 Antibody detects level of Na+/K+/Cl-Cotransporter 2 & has been published & validated for use in ELISA & WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Western blot: 1-10 μg/mL using ECL. Full length human NKCC2 is ~150 kDa.



ELISA: 0.5-1 μg/mL using 1 μg/mL control peptide per well.

Optimal working dilutions must be determined by the end user.

Recognizes rat Sodium, Potassium, Chloride Cotransporter 2 (NKCC2, BSC1, CCC2). The immunogen shows no significant sequence homology with NKCC1 or TSC.

SPECIES REACTIVITIES: The immunogen sequence is 100% conserved in mouse, 87% in human and rabbit. Reactivity with other species has not been confirmed.

Affinity purified immunoglobulin. Liquid in PBS containing 0.1% BSA.

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.