AB3562P
Anti-Na+/K+/Cl- Cotransporter 2 Antibody
Chemicon®, from rabbit
Synonym(s):
NKCC2
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-Na+/K+/Cl- Cotransporter 2 Antibody, Chemicon®, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SLC12A1(6557)
Application
Anti-Na+/K+/Cl-Cotransporter 2 Antibody detects level of Na+/K+/Cl-Cotransporter 2 & has been published & validated for use in ELISA & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Western blot: 1-10 μg/mL using ECL. Full length human NKCC2 is ~150 kDa.
ELISA: 0.5-1 μg/mL using 1 μg/mL control peptide per well.
Optimal working dilutions must be determined by the end user.
ELISA: 0.5-1 μg/mL using 1 μg/mL control peptide per well.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Recognizes rat Sodium, Potassium, Chloride Cotransporter 2 (NKCC2, BSC1, CCC2). The immunogen shows no significant sequence homology with NKCC1 or TSC.
SPECIES REACTIVITIES: The immunogen sequence is 100% conserved in mouse, 87% in human and rabbit. Reactivity with other species has not been confirmed.
SPECIES REACTIVITIES: The immunogen sequence is 100% conserved in mouse, 87% in human and rabbit. Reactivity with other species has not been confirmed.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
A 15 amino acid peptide sequence near the C-terminus of rat NKCC2 (Haas et al. 1987; Gamba et al. 1994; Simon et al. 1996; Mount et al. 1995; Igarashi et al. 1995; Mount et al. 1998). The immunogen peptide is available (catalog number AG205) for use in pre-absortion controls.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Affinity purified immunoglobulin. Liquid in PBS containing 0.1% BSA.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Nobuhiro Ayuzawa et al.
Scientific reports, 12(1), 20310-20310 (2022-11-27)
Development of the renal medulla continues after birth to form mature renal papilla and obtain urine-concentrating ability. Here, we found that a small GTPase, Rac1, plays a critical role in the postnatal development of renal papilla. Mice with distal tubule-specific
Matteo Trudu et al.
Nature medicine, 19(12), 1655-1660 (2013-11-05)
Hypertension and chronic kidney disease (CKD) are complex traits representing major global health problems. Multiple genome-wide association studies have identified common variants in the promoter of the UMOD gene, which encodes uromodulin, the major protein secreted in normal urine, that
Dendritic compartmentalization of chloride cotransporters underlies directional responses of starburst amacrine cells in retina.
Gavrikov, KE; Nilson, JE; Dmitriev, AV; Zucker, CL; Mangel, SC
Proceedings of the National Academy of Sciences of the USA null
Agnieszka Konopacka et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(13), 5144-5155 (2015-04-04)
The Na-K-2Cl cotransporter 2 (NKCC2) was thought to be kidney specific. Here we show expression in the brain hypothalamo-neurohypophyseal system (HNS), wherein upregulation follows osmotic stress. The HNS controls osmotic stability through the synthesis and release of the neuropeptide hormone
Serena Milano et al.
Journal of cellular and molecular medicine, 28(8), e18301-e18301 (2024-04-23)
X-linked nephrogenic diabetes insipidus (X-NDI) is a rare congenital disease caused by inactivating mutations of the vasopressin type-2 receptor (AVPR2), characterized by impaired renal concentrating ability, dramatic polyuria, polydipsia and risk of dehydration. The disease, which still lacks a cure
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