Anti-Growth Associated Protein 43 Antibody

23.6 kDa

GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.

Purified GAP-43 from feline brain (McIntosh and Parkinson, 1990).

Immunocytochemistry: 1:100-1:400 for mouse primary neurons, NTera2 neurons.

Immuohistochemistry: 1:2,000 on coronal sections of adult visual cortex (McIntosh and Parkinson, 1990). AB5312 has been used to stain GAP-43 in 4% paraformaldehyde-fixed paraffin-embedded tissue sections of mouse olfactory bulb (Legrier, 2001).

Western blot: 1:500 - 1:1,000. Detects a single band at approximately 50 kDa on western blots of protein extracts from adult visual cortex (McIntosh and Parkinson, 1990).

Immunoprecipitation

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neuroregenerative Medicine

Signaling Neuroscience

This Anti-Growth Associated Protein 43 Antibody is validated for use in IC, IH, IH(P), IP, WB for the detection of Growth Associated Protein 43.

AB5312 specifically recognizes GAP-43 regardless of the protein′s phosphorylation state. This antiserum has been refered to in the literature as CG-1.

Serum. Liquid with 0.02% sodium azide added as a preservative.

Unpurified

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Rat dorsal root ganglion tissue that has been subjected to a spinal nerve ligation, cultured neurons

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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