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Merck
CN

AB5447

Anti-Tropomyosin Antibody, 5NM1 and 5NM2

Chemicon®, from sheep

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-Tropomyosin Antibody, 5NM1 and 5NM2, Chemicon®, from sheep

biological source

sheep

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NM_001043351.1
NM_001043352.1
NM_001043353.1
NM_152263.2
NM_153649.3

UniProt accession no.

P06753

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... TPM3(7170)

Application

Research Sub Category
Muscle Physiology
Detect Tropomyosin using this Anti-Tropomyosin Antibody, 5NM1 & 5NM2 validated for use in WB, IC, IH.
Research Category
Metabolism
Western Blot: 1:100-1:500. Antibody reacts with a band at 29-30kDa.

Immunocytochemistry: 1:100-1:500

Immunohistochemistry: 1:100-1:500

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Tropomyosin 5NM1 and 5NM2.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: 5NM1 and 5NM2
Synthetic peptide corresponding to the 9d exon from the gamma gene of tropomyosin.

Physical form

Affinity purified immunoglobulin in PBS (liquid).

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sawako Yamashiro et al.
The Journal of biological chemistry, 289(17), 11616-11629 (2014-03-20)
Tropomodulins (Tmods) are F-actin pointed end capping proteins that interact with tropomyosins (TMs) and cap TM-coated filaments with higher affinity than TM-free filaments. Here, we tested whether differences in recognition of TM or actin isoforms by Tmod1 and Tmod3 contribute
David S Gokhin et al.
Molecular biology of the cell, 26(9), 1699-1710 (2015-02-27)
Short, uniform-length actin filaments function as structural nodes in the spectrin-actin membrane skeleton to optimize the biomechanical properties of red blood cells (RBCs). Despite the widespread assumption that RBC actin filaments are not dynamic (i.e., do not exchange subunits with
Functional effects of mutations in the tropomyosin-binding sites of tropomodulin1 and tropomodulin3.
Lewis, RA; Yamashiro, S; Gokhin, DS; Fowler, VM
Cytoskeleton (Hoboken, N.J.) null
David S Gokhin et al.
The Journal of cell biology, 194(1), 105-120 (2011-07-06)
The sarcoplasmic reticulum (SR) serves as the Ca(2+) reservoir for muscle contraction. Tropomodulins (Tmods) cap filamentous actin (F-actin) pointed ends, bind tropomyosins (Tms), and regulate F-actin organization. In this paper, we use a genetic targeting approach to examine the effect
Maria Sckolnick et al.
Molecular biology of the cell, 27(19), 2889-2897 (2016-08-19)
Tropomyosin (Tpm) isoforms decorate actin with distinct spatial and temporal localization patterns in cells and thus may function to sort actomyosin processes by modifying the actin track affinity for specific myosin isoforms. We examined the effect of three Tpm isoforms
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