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AB5471P

Sigma-Aldrich

Anti-Semaphorin 3F Antibody

Chemicon®, from rabbit

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100 μG
CN¥4,753.86

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

CN¥4,753.86

Estimated to ship onSeptember 15, 2025Details

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biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
western blot: suitable

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Anti-Semaphorin 3F Antibody Chemicon®, from rabbit

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Anti-Semaphorin 3A Antibody, central region Chemicon®, from rabbit

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Anti-Semaphorin 3A Antibody, central region

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

affinity purified immunoglobulin

UniProt accession no.

Q13275

UniProt accession no.

Q13275

UniProt accession no.

Q969G3

UniProt accession no.

Q14563

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: 1:200- 1:500

technique(s)

affinity binding assay: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

western blot: suitable

clone

polyclonal

clone

polyclonal

clone

1C6, recombinant monoclonal

clone

polyclonal

General description

90 kDa

Immunogen

Synthetic peptide from human Semaphorin 3F (Catalog number AG363). (TPWTQTQAVRFRFSRATDGALRPM).

Application

Anti-Semaphorin 3F Antibody is an antibody against Semaphorin 3F for use in IC & WB.
Research Category
Neuroscience
Research Sub Category
Growth Cones & Axon Guidance
Western blot: 0.5-1.0 μg/mL using ECL. Suggested antibody dilution buffer is TBST (10 mM Tris-HCl, pH 8.0, 150 mM NaCl with 0.05% Tween 20). Suggested incubation is for two hours at room temperature or overnight at 2-8°C.
Immunocytochemistry.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Semaphorin 3F (Sema3F), recombinant and purified protein. By Western blot the antibody recognizes a protein of 90 kDa in total extracts from tissue culture cells producing Sema3F. Semaphorin 3F is expressed ubiquitously with higher levels in lung, brain, spleen, muscle and heart.
This antibody may also react with mouse because of extremely high sequence conservation in the SEM3F protein.

Physical form

Affinity Purified immunoglobulin. Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.
ImmunoAffinity Purified

Preparation Note

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Embryonic brain tissue, neural growth cones

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB5471

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

    • Certificates of Analysis (COA)

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    Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, 24(2), 120-128 (2011-11-19)
    Nerve fibers can exert trophic/anti-trophic effects on epithelial cells. Substance P (SP) is a pro-proliferative neuropeptide, whereas sympathetic noradrenaline is anti-proliferative at high concentrations. Density of noradrenergic and sensory nerve fibers and presence of nerve repellent factors specific for noradrenergic
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    International journal of molecular sciences, 17(1) (2016-01-20)
    Breast cancer is the most common tumor and the second leading cause of cancer death among woman, mainly caused by the metastatic spread. Tumor invasiveness is due to an altered expression of adhesion molecules. Among them, semaphorins are of peculiar

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