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AB5543

Sigma-Aldrich

Anti-MAP2 Antibody

Chemicon®, from chicken

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Synonym(s):
Anti-MAP-2, Anti-MAP2A, Anti-MAP2B, Anti-MAP2C
eCl@ss:
32160702
NACRES:
NA.41

biological source

chicken

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NM_001039538.1
NM_002374.3
NM_031845.2
NM_031847.2

UniProt accession no.

P11137

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

rat ... Map2(25595)

Specificity

Microtubule Associated Protein 2 (MAP2). It is expected that the antibody will react with all mammals.

Immunogen

Purified bovine brain MAP2

Application

Anti-MAP2 Antibody is an antibody against MAP2 for use in IC, IH & WB.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Neurofilament & Neuron Metabolism
Western blot: 1:5,000-1:10,000 using ECL. Recognizes a doublet of ~280 kDa and a doublet of ~70kDa.

Immunohistochemistry on frozen tissue sections. Suggested fixative is 4% paraformaldehyde (1-10 minutes) followed by 5 minutes in -20°C methanol.

Immunocytochemistry: 1:5,000-1:10,000 on cells in tissue culture fixed for 1-10 minutes in 10% formalin followed by five minutes in -20°C methanol.

Optimal working dilutions must be determined by the end user.

Target description

Recognizes a doublet of ~280 kDa and a doublet of ~70kDa

Physical form

Ammonium Sulfate Precipitation
Format: Purified
Purified chicken IgY. Liquid in PBS containing 5 mM sodium azide.

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive = cerebral cortex. Negative = liver or kidney.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

recommended

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

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Stimulation of Glia Reveals Modulation of Mammalian Spinal Motor Networks by Adenosine.
Acton, D; Miles, GB
Testing null
Yumiko V Taguchi et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(40), 9617-9631 (2017-08-30)
Glucocerebrosidase 1 (GBA) mutations responsible for Gaucher disease (GD) are the most common genetic risk factor for Parkinson's disease (PD). Although the genetic link between GD and PD is well established, the underlying molecular mechanism(s) are not well understood. We
Riikka H Hämäläinen et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(38), E3622-E3630 (2013-09-05)
Mitochondrial DNA (mtDNA) mutations manifest with vast clinical heterogeneity. The molecular basis of this variability is mostly unknown because the lack of model systems has hampered mechanistic studies. We generated induced pluripotent stem cells from patients carrying the most common
Ester Orav et al.
eNeuro, 4(3) (2017-07-07)
Kainate-type glutamate receptors (KARs) are highly expressed in the developing brain, where they are tonically activated to modulate synaptic transmission, network excitability and synaptogenesis. NETO proteins are auxiliary subunits that regulate biophysical properties of KARs; however, their functions in the
J A Fantuzzo et al.
eNeuro, 4(6) (2017-12-09)
Synapse formation analyses can be performed by imaging and quantifying fluorescent signals of synaptic markers. Traditionally, these analyses are done using simple or multiple thresholding and segmentation approaches or by labor-intensive manual analysis by a human observer. Here, we describe
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