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Merck
CN

AB7144F

Anti-Fibrinogen Antibody, FITC conjugated

Chemicon®, from sheep

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-Fibrinogen Antibody, FITC conjugated, Chemicon®, from sheep

technique(s)

western blot: suitable
immunodiffusion: suitable
immunofluorescence: suitable

biological source

sheep

conjugate

FITC conjugate

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

manufacturer/tradename

Chemicon®

NCBI accession no.

NM_000508.3
NM_021871.2

UniProt accession no.

P02671

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... FIBCD1(84929)

Application

Detect Fibrinogend using this Anti-Fibrinogen Antibody, FITC conjugated validated for use in IF, ID & WB.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors
WORKING DILUTION

The final working dilution is dependent on the specific assay conditions. However, in fluorescence microscopy good results can be expected with dilutions in the following ranges:

1. Incident light excitation

Direct immunofluorescence: 1/10 - 1/80

Indirect immunofluorescence: 1/20 - 1/400

2. Transmitted light excitation

Direct immunofluorescence: 1/5 - 1/40

Indirect immunofluorescence: 1/10 - 1/80

Biochem/physiol Actions

Prior to conjugation, the antibody was specific for fibrinogen when tested against human plasma by Coomassie Blue R stained double immunodiffusion and immunoelectrophoresis. Undesired immune reactivity has been removed by solid phase immunoadsorption.

LABELLING RATIO

Fluorescein:antibody molar ratio: 2.8 - 3.3

Absorbance ratio (A493/A276): 0.78 + 0.05

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Purified fibrinogen derived from pooled human plasma.

Physical form

The antibody is supplied in 20mM Tris, 70mM NaCl, pH 8.0, with 0.1% sodium azide as preservative.

Preparation Note

Store at +2 to +8°C, for up to 6 months, undiluted. Protect from light.

WARNING: This reagent contains 0.1% sodium azide as preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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