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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat, mouse

species reactivity (predicted by homology)

rhesus macaque (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

NP_116274

UniProt accession no.

Q86TL0

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ATG4D(84971)

General description

Atg4D (autophagy-related protein 4D) is a member of the Atg4 family of C54 endopeptidases that are required for the synthesis of autophagosomes. Atg4D is found to be cleaved by caspase-3 into a more active form where it can interact with the autophagosome marker Atg8. Atg4D is also known to be associated with mitochondrial dysfunction and the induction of apoptosis.

Specificity

This antibody recognizes Atg4D at the N-terminus.

Immunogen

Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of human Atg4D.

Application

Anti-Atg4D Antibody is an antibody against Atg4D for use in WB, IH(P).
Immunohistochemistry (Paraffin) Analysis: A 1:200 dilution of a previous lot detected Atg4D in human testicular cancer tissue.

Western Blot Analysis: 1 µg/mL from a representative lot detected Atg4D on 10 µg of human fetal skeletal muscle tissue, NIH/3T3, and L6 cell lysates.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected Atg4D in 10 µg of A431 cell lysate.

Target description

~56 kDa observed.
The calculated molecular weight is 53 kDa; however, Atg4D has been shown as a ~55 kDa band in western blots (Li, M., et al. (2011). J Biol Chem. 286(9):7327-7338.)

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
A431 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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