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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IHC, WB
Citations:
-
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse
species reactivity (predicted by homology)
human (immunogen homology)
technique(s)
immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... BCL2A1(597)
General description
Protein BFL-1, also known as protein GRS, Bcl-2-like protein 5 (Bcl2-L-5), and Bcl-2-related protein A1 (BCL2A1), is a member of the Bcl-2 family. BFL-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. BFL-1 is expressed in peripheral blood, spleen, bone marrow, lung, small intestine, and testis.
~39 kDa observed. Uniprot describes two isoforms at ~19 kDa and ~20 kDa
Immunogen
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of human BFL-1.
Application
Anti-BFL-1 Antibody is a highly specific rabbit polyclonal antibody, that targets Bcl-2-related protein A1 & has been tested in western blotting, IHC & Immunofluorescence.
Immunohistochemistry Analysis: 2 µg/mL from a representative lot detected BFL-1 in mouse kidney tissue.
Immunofluorescence Analysis: 20 µg/mL from a representative lot detected BFL-1 in mouse kidney cells.
Immunofluorescence Analysis: 20 µg/mL from a representative lot detected BFL-1 in mouse kidney cells.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Biochem/physiol Actions
This antibody recognizes the N-terminus of BFL-1.
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse kidney tissue lysate
Mouse kidney tissue lysate
Evaluated by Western Blotting in mouse kidney tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected BFL-1 in 15 µg of mouse kidney tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected BFL-1 in 15 µg of mouse kidney tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| ABC339 | 04053252901362 |