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Merck
CN

ABC937

Anti-FOXL1 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Forkhead box protein L1, Forkhead-related protein FKHL11, Forkhead-related transcription factor 7, FREAC-7, FOXL1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunohistochemistry
western blot
Species reactivity:
human
Citations:
1
Technique(s):
immunohistochemistry: suitable (paraffin)
western blot: suitable
Uniprot accession no.:
Q12952

Key Documents

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Product Name

Anti-FOXL1 Antibody, from rabbit, purified by affinity chromatography

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

sheep (based on 100% sequence homology), feline (based on 100% sequence homology), porcine (based on 100% sequence homology), bovine (based on 100% sequence homology), horse (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

NP_005241

UniProt accession no.

Q12952

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... FOXL1(2300)

Analysis Note

Evaluated by Western Blotting in human colon tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected FOXL1 in 10 µg of human colon tissue lysate.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected FOXL1 in human breast cancer tissue.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-FOXL1 Antibody is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of FOXL1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Forkhead box protein L1 (UniProt Q12952; also known as Forkhead-like 11, Forkhead-related protein FKHL11, Forkhead-related transcription factor 7, FREAC-7) is encoded by the FOXL1 (also known as FKHL11, FREAC7) gene (Gene ID 2300) in human. FOXL1 protein belongs to the forehead box (Fox) family of transcription factors characterized by a common DNA-binding domain (the forkhead box). FOXL1 is involved in regulating the proliferation of gastrointestinal epithelial cells. Loss of Foxl1 leads to distorted architecture of the stomach and small intestine in mice due to a markedly increased epithelial proliferation. Foxl1 knockout is correlated with an enhanced Wnt/β-catenin pathway activation as evidenced by increased β-catenin nuclear localization. FOXL1 expression is down-regulated in the majority of human clear cell renal cell carcinoma (ccRCC), at both mRNA and protein levels. FOXL1 expression inversely correlates with the aggressive phenotype of ccRCC and the survival outcome of patients.
~36 kDa observed. Uncharacterized band(s) may appear in some lysates.

Immunogen

KLH-conjugated linear peptide corresponding to human FOXL1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hiroki Kobayashi et al.
Gastroenterology, 160(4), 1224-1239 (2020-11-17)
Cancer-associated fibroblasts (CAFs), key constituents of the tumor microenvironment, either promote or restrain tumor growth. Attempts to therapeutically target CAFs have been hampered by our incomplete understanding of these functionally heterogeneous cells. Key growth factors in the intestinal epithelial niche

Related Content

White Paper: Further considerations of antibody validation and usage.
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System

A major focus of breast cancer research is to understand the mechanisms responsible for disease progression and drug resistance. Toward that end, it has been found that approximately two thirds of all human breast carcinomas overexpress the Estrogen Receptor α (ERα) protein and it remains the primary pharmacological target for endocrine therapy1,2. The normal cellular function of ERα is as a transcription factor that mediates a wide variety of physiological processes, many of which are dependent upon phosphorylation of the receptor at specific amino acid residues3,4. Indeed, ERα is known to be phosphorylated at a multitude of different sites, yet how these all correlate to disease remains unclear5. Here, we interrogated multiple sites of ERα for phosphorylation status by screening an extensive panel of different breast cancer patient samples and other non-breast cancer tissue microarray (TMA) slide samples to determine their relevance to disease.

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