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ABD69

Anti-Nestin, Human Antibody

Name: Anti-Nestin, Human Antibody
Brand: MM

serum, from rabbit

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Synonym(s):

Nestin

eCl@ss:

32160702

NACRES:

NA.41

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

should not react with

rat, mouse

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NP_006608

UniProt accession no.

P48681

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NES(10763)

General description

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.

Specificity

This antibody recognizes human Nestin. Antibody is not reactive with rat and mouse Nestin protein.

Immunogen

GST-tagged recombinant protein corresponding to human Nestin.

Application

Detect Nestin, Human using this Anti-Nestin, Human Antibody validated for use in Western Blotting, ICC & IHC.

Evaluated by Immunocytochemistry in human WI38 cells.
Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected Nestin in human WI38 cells.
Evaluated by Immunohistochemistry in human glioblastoma tissue.
Immunocytochemsitry Analysis: A 1:2,000 dilution of this antibody detected Nestin in human glioblastoma tissue.
Evaluated by Western Blot in human U251 glioblastoma cells. Western Blot Analysis: A 1:25,000 dilution of this antibody has been shown to detected Nestin in human U251 glioblastoma cells.

Quality

Evaluated by Immunocytochemistry in human U251 glioblastoma cells. Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected Nestin in human U251 glioblastoma cells.

Target description

The anti-Nestin antibody has an observed MW at 220kDa Antibody may see 260kDa band in some cells and/or treatments

Linkage

Replaces: AB5922

Analysis Note

Control
U251 glioblastoma cells.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

ZRB69

Anti-Nestin Antibody, clone 8B8, ZooMAb^® Rabbit Monoclonal, recombinant, expressed in HEK 293 cells

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

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Suppression of STAT3 NH2 -terminal domain chemosensitizes medulloblastoma cells by activation of protein inhibitor of activated STAT3 via de-repression by microRNA-21.

Sutapa Ray et al.

Molecular carcinogenesis, 57(4), 536-548 (2017-12-28)

Medulloblastoma (MB) is a malignant pediatric brain tumor with poor prognosis. Signal transducers and activators of transcription-3 (STAT3) is constitutively activated in MB where it functions as an oncoprotein, mediating cancer progression and metastasis. Here, we have delineated the functional

Aberrant mitochondrial function in patient-derived neural cells from CDKL5 deficiency disorder and Rett syndrome.

Smita Jagtap et al.

Human molecular genetics, 28(21), 3625-3636 (2019-09-14)

The X-linked neurodevelopmental diseases CDKL5 deficiency disorder (CDD) and Rett syndrome (RTT) are associated with intellectual disability, infantile spasms and seizures. Although mitochondrial dysfunction has been suggested in RTT, less is understood about mitochondrial function in CDD. A comparison of

Intraspinal transplantation of mouse and human neural precursor cells.

Weinger, JG; Chen, L; Coleman, R; Leang, R; Plaisted, WC; Loring, JF; Lane, TE

Current Protocols in Stem Cell Biology null

Electrical preconditioning of stem cells with a conductive polymer scaffold enhances stroke recovery.

Paul M George et al.

Biomaterials, 142, 31-40 (2017-07-19)

Exogenous human neural progenitor cells (hNPCs) are promising stroke therapeutics, but optimal delivery conditions and exact recovery mechanisms remain elusive. To further elucidate repair processes and improve stroke outcomes, we developed an electrically conductive, polymer scaffold for hNPC delivery. Electrical

Significance of Glioma Stem-Like Cells in the Tumor Periphery That Express High Levels of CD44 in Tumor Invasion, Early Progression, and Poor Prognosis in Glioblastoma.

Masahiro Nishikawa et al.

Stem cells international, 2018, 5387041-5387041 (2018-09-14)

Glioblastoma multiforme (GBM) is the most aggressive malignant brain tumor and a subpopulation of glioma stem-like cells (GSCs) is likely responsible for the invariable recurrence following maximum resection and chemoradiotherapy. As most GSCs that are located in the perivascular and

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