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Merck
CN

ABD99

Anti-Rex-1 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Zinc finger protein 42, Reduced expression protein 1, mREX-1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, WB
Citations:
-

Key Documents

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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

immunocytochemistry: suitable, western blot: suitable

NCBI accession no.

NP_777560

UniProt accession no.

Q96MM3

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... ZFP42(132625)

General description

The Rex-1 (Zfp-42) gene, which encodes an acidic zinc finger protein, is expressed at high levels in embryonic stem (ES) and F9 teratocarcinoma cells. The Rex-1 promoter is regulated by specific octamer family members in early embryonic cells, and is involved in retinoic acid (RA)-associated reduction in expression. Rex-1 is Involved in self-renewal property of ES cells and may be involved in transcriptional regulation of pluripotency signaling pathways. Rex-1 is also involved in the reprogramming of X-chromosome inactivation during the acquisition of pluripotency and is required for efficient elongation of TSIX, a non-coding RNA antisense to XIST.
~35 kDa observed. Uncharacterized band(s) may appear in some lysates.

Immunogen

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of Human Rex-1.

Application

Detect Rex-1 using this Anti-Rex-1 Antibody validated for use in western blotting & ICC.
Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Rex-1 in a undifferentiated H9 human embryonic stem cells.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Physical form

Antigen Affinity Purified
Purified rabbit Polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Undifferentiated H9 human embryonic stem cells.
Evaluated by Western Blotting in a undifferentiated H9 human embryonic stem cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Rex-1 in undifferentiated H9 human embryonic stem cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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