ABE1331
Anti-Zinc finger protein Eos Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Zinc finger protein Eos, Ikaros family zinc finger protein 4
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, WB
Citations:
1
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
mouse (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... IKZF4(64375)
General description
Zinc finger protein Eos, also known as Zinc finger protein Eos, or Ikaros family zinc finger protein 4, and encoded by the gene name IKZF4/ KIAA1782/ ZNFN1A4, is a member of the Ikaros C2H2-type zinc-finger protein family. Zinc finger protein Eos is a DNA-binding protein that binds to the 5′GGGAATRCC-3′ Ikaros-binding sequence. It is also a transcriptional repressor, which interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. Zinc finger protein Eos may be involved in the development of central and peripheral nervous systems. Zinc finger protein Eos interacts with other family members: IKZF1, IKZF2, IKZF3 AND IKZF5; as well as CTBP2, SPI1 and MITF. Zinc finger protein Eos is highly expressed in skeletal muscle, and with low levels of expression in heart, thymus, kidney, liver, and spleen. Zinc finger protein Eos is also expressed in the hematopoietic cell lines MOLT-4, NALM-6 and K-562. High level of expression has been detected in THP-1 and M-07e cell lines, which have characteristics of myeloid and early megakaryocytic cells respectively.
~60 kDa observed. This antibody is predicted to detect both isoforms of Zinc finger protein Eos (~64 & ~59 kDa) based on immunogen sequence. Uncharacterized band(s) may appear in some lysates.
Immunogen
KLH-conjugated linear peptide corresponding to human Zinc finger protein Eos near the C-terminus.
Application
This Anti-Zinc finger protein Eos antibody is validated for use in WB, IC for the detection of Zinc finger protein Eos.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Zinc finger protein Eos in 10 µg of human thymus tissue lysate.
Immunocytochemistry Analysis: 1.0 µg/mL from a representative lot detected Zinc finger protein Eos in HepG2 cells.
Immunocytochemistry Analysis: 1.0 µg/mL from a representative lot detected Zinc finger protein Eos in HepG2 cells.
Biochem/physiol Actions
Immunogen sequence is 93% homologous to Bovine.
Analysis Note
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Zinc finger protein Eos in 10 µg of HepG2 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Zinc finger protein Eos in 10 µg of HepG2 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Kaitlin A Read et al.
Journal of immunology (Baltimore, Md. : 1950), 199(7), 2377-2387 (2017-08-30)
B cell lymphoma-6 (Bcl-6) is a transcriptional repressor that is required for the differentiation of T follicular helper (TFH) cell populations. Currently, the molecular mechanisms underlying the transcriptional regulation of Bcl-6 expression are unclear. In this study, we have identified
Global Trade Item Number
| SKU | GTIN |
|---|---|
| ABE1331 | 04055977117011 |