Ligand-dependent corepressor (UniProt: Q96JN0; also known as LCoR, Mblk1-related protein 2) is encoded by the LCOR (also known as C10orf12, KIAA1795, MLR2) gene (Gene ID: 84458) in human. LCoR is a ubiquitously distributed protein in fetal and adult tissues and serves as a transcriptional co-repressor that is recruited to agonist-bound nuclear receptors through a single LxxLL motif at the N-terminal region. It may also act as a transcription activator that binds DNA elements with the sequence 5′-CCCTATCGATCGATCTCTACCT-3′. Three isoforms of LCoR have been described that are produced by alternative splicing. This polyclonal antibody is raised against a large isoform (isoform 3; a splice variant), which associates with the PRC2 complex through its C-terminal region. Its nuclear localization signal is reported to be in amino acids 339-345 and it reacts with nuclear receptors via amino acids 53-57. (Ref.: Conway, E., et al. (2018). Mol. Cell 70(3); 408-421).

~250 kDa observed; 170.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Epitope: unknown

KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal half of human Ligand-dependent corepressor (LCoR), isoform 3.

Anti-LCoR, Cat. No. ABE1367, is a highly specific rabbit polyclonal antibody that targets a splice variant form of Ligand-dependent corepressor (LCoR) and has been tested in Immunocytochemistry and Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: A 1:500 dilution from a representative lot detected LCoR in whole cell lysates from Pali1 KO and matched wild type mouse embryonic stem (Courtesy of Dr. Eric Conway, Dr. Emilia Jerman & Prof. Adrian P. Bracken at the Smurfit Institute of Genetics, Trinity College Dublin, Ireland).

Western Blotting Analysis: A representative lot detected LCoR in Western Blotting applications (Conway, E., et. al. (2018). Mol. Cell. 70(3):408-421).

This rabbit polyclonal antibody detects a splice variant of human and murine Ligand-dependent corepressor (LCoR). It targets an epitope with in the N-terminal half.

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Immunocytochemistry in NTERA2 cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected LCoR in NTERA2 cells.

Concentration: Please refer to lot specific datasheet.

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