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ABE1372

Sigma-Aldrich

Anti-OGG1 Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
N-glycosylase/DNA lyase, 1.8-oxoguanine DNA glycosylase, DNA-(apurinic or apyrimidinic site) lyase, AP lyase
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... OGG1(4968)

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This Item
ABE1440PRS3863HPA006531
Anti-OGG1 Antibody from rabbit, purified by affinity chromatography

Sigma-Aldrich

ABE1372

Anti-OGG1 Antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

human

species reactivity

mouse, human

species reactivity

rat, human, mouse

species reactivity

human

technique(s)

western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:50-1:200

NCBI accession no.

NP_002533

NCBI accession no.

NP_003202

NCBI accession no.

-

NCBI accession no.

-

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General description

The protein named N-glycosylase/DNA lyase or alternatively, 8-oxoguanine (8-OxoG) DNA glycosylase or AP lyase, and encoded by the gene named OGG1/MMH/MUTM/OGH1, is a DNA repair enzyme that incises DNA at 8-oxoG residues and excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. OGG1 also has beta-lyase activity that nicks DNA 3′ to the lesion. OGG1 is localized to the nucleus and nucleoplasm; in UVA-irradiated cells it can be found in the nuclear speckles. There is also an isoform found in the mitochondrion. Interestingly, OGG1 appears to be play a critical role in repairing mtDNA damage; mtDNA damage is linked to mitochondrial dysfunction, increased oxidative stress, and insulin resistance in muscle cells, and studies using OGG1 knockouts or over expressing OGG1 demonstrate that OGG1 is important for proper mtDNA damage repair and that disruption of OGG1 can lead to increased mtROS (reactive oxygen species) production and subsequent regulation of downstream events leading to insulin resistance in skeletal muscle. OGG1, or rather defects in OGG1 function, are also associated with a number of carcinomas, particularly Renal Cell Carcinomas of various cell types.

Immunogen

Recombinant protein corresponding to human OGG1.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-OGG1 Antibody is validated for use in Western Blotting for the detection of OGG1.
Western Blotting Analysis: A representative lot detected recombinant protein OGG1 (Bjoras, M., et al. (1997). EMBO. 16(20):6314–6322).
Western Blotting Analysis: A representative lot detected OGG1 in Melphalan-resistant cells (Sousa, M., et al. (2013). PLOS One. 8(2):e55493).

Quality

Evaluated by Western Blotting in recombinant protein OGG1.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant protein OGG1 in 1 µg of cell lysate.

Target description

~38 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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