Anti-H3K4me3Q5amphetaminyl

Histone H3.1 (UniProt: P68431; also known as Histone H3/a, 3/b,3/c, 3/d, 3/e, 3/f, 3/h, 3/i, 3/j, 3/k, 3/l) is encoded by the H3C1 gene (Gene ID: 8350) in human. Histones are highly conserved basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. They play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The amino-terminal tails of histone proteins are subject to posttranslational modifications, including acetylation and methylation, which recruit downstream regulatory factors, influence chromatin structure, and are critical determinants of transcription. Histone H3Q5amphetaminylation is a novel post-translational modification, where the glutamine-5 residue of histone H3 becomes covalently modified by amphetamine (a central nervous system stimulant which is commonly used to treat ADHD and narcolepsy) via transglutaminase 2 (TG2)-mediated incorporation. This modification is structurally similar to histone dopaminylation, which has been implicated in transcriptional regulation and neuronal function. Given the psychoactive properties of amphetamine and it′s long-term use by patients, H3Q5 amphetaminylation could in theory influence gene expression patterns in a drug-specific manner, potentially affecting neuronal plasticity, addiction-related pathways, and other neuronal processes. Amphetaminylation of glutamine 5 on histone H3 is recently identified as a permissive post-translational modification that coexists with adjacent lysine 4 trimethylation.  Characterization of this modification could provide insight into how amphetamines modify long-term epigenetics in the brain and body, with implications for neuropsychiatric disorders, substance use research, and more.

KLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Histone H3.1 modified with tri-methylation at lysine 4 and amphetamine at glutamine 5 position.

Quality Control Testing

Evaluated by Western Blotting in Recombinant TG2, Recombinant mononucleosomes reacted with amphetamine.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Histone H3.1 modified with amphetamine at Glutamine 5, in Recombinant TG2 + Recombinant mononucleosomes reacted with 5mM amphetamine.

This rabbit polyclonal antibody specifically detects Histone H3.1 modified with tri-methylation at lysine 4 and amphetamine at glutamine 5 position.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

0.15 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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