Anti-ZNF189

Zinc finger protein 189 (UniProt: Q8BKP2; also known as ZNF189, ZFP189) is encoded by the Zfp189 gene (Gene ID: 230162) in murine species. ZFP189 is a highly conserved member of the Krüppel-associated box (KRAB)-containing group of zinc finger proteins and is present in the nucleus of all cell types. It is involved in the regulation of transcription by RNA polymerase II. It contains 16 C2H2 type zinc finger domains and a KRAB domain at its N-terminal (aa 1-75) that is divided into box A and B. Box A is essential for transcriptional repression. Overexpression of Zfp189 gene in prefrontal cortical neurons is shown to preferentially activate gene network that is unique to resilience in mice. CREB is predicted to be its upstream regulator that binds to the zfp189 promoter. (Ref.: Lorsch, ZS et al. (2019). Nature Neurosci. 22(9); 1413-1423; Odeberg, J. et al. (1998). Genomics. 50(2); 213-221).

This rabbit polyclonal antibody detects Zinc finger protein 189. It targets an epitope within 14 amino acids from the N-terminal half.

KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal half of mouse Zinc finger protein 189.

Quality Control Testing

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ZNF189 in U2OS cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ZNF189 in mouse and rat brain tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected ZNF189 in mouse brain tissue sections.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ZNF189 in mouse nucleus accumbens (Courtesy of Dr. Nestler Lab @ Mount Sinai New York).

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected ZNF189 in U2OS cell line.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-ZNF189, Cat. No. ABE2917, is a rabbit polyclonal antibody that detects Zinc finger protein 189 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at +2°C to +8°C from date of receipt.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.