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ABE543

Sigma-Aldrich

Anti-PRDM16 Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
PR domain zinc finger protein 16, PR domain-containing protein 16, Transcription factor MEL1, MDS1/EVI1-like gene 1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

(Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide)

mol wt

(Target molecular weight ~140 kDa observed; 140.25 kDa calculated. Uncharacterized bands may be observed in some lysate(s).)

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

horse (based on 100% sequence homology), canine (based on 100% sequence homology), bovine (based on 100% sequence homology), nonhuman primates (based on 100% sequence homology), guinea pig (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable ((Paraffin))
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NP_071397

UniProt accession no.

Q9HAZ2

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PRDM16(63976)

General description

Anti-PRDM16, Cat. No. ABE543, is a rabbit polyclonal antibody that detects PR domain zinc finger protein 16 (PRDM16) and is teste for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunogen

KLH-conjugated linear peptide corresponding to human PRDM16.

Application

Detect PRDM16 using this rabbit polyclonal antibody, Anti-PRDM16 Antibody validated for use in western blotting, IP & IHC.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated PRDM16 from 500 µg of K562 nuclear extract.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected PRDM16 in human kidney tissues.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

Quality

Evaluated by Western Blotting in LNCaP nuclear extract.

Western Blotting Analysis: 2 µg/mL of this antibody detected PRDM16 in 10 µg of LNCaP nuclear extract.

Target description

~140 kDa observed. Four isoforms are known to exist. This protein has been observed at 150 kDa, 135 kDa, and 100 kDa (EMD Millipore Cat. No. ABD130). Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Affinity purified
Purified rabbit serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Liang-Ru Fei et al.
Journal of experimental & clinical cancer research : CR, 38(1), 35-35 (2019-01-27)
The transcription factor PR domain containing 16 (PRDM16) is known to play a significant role in the determination and function of brown and beige fat. However, the role of PRDM16 in tumor biology has not been well addressed. Here we

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