Pre-mRNA-splicing regulator WTAP (UniProt Q15007; also known as Female-lethal (2)D homolog, hFL(2)D, Wilms tumor 1-associating protein, WT1-associated protein) is encoded by the WTAP (also known as KIAA0105) gene (Gene ID 9589) in human. WTAP is an ubiquitously expressed nuclear protein that is localized throughout the nucleoplasm and in speckles and partially co-localizes with splicing factors. A mouse homolog has also been reported that shares 96% sequence identity. WTAP regulates G2/M cell-cycle transition by binding to the 3′ UTR of CCNA2, which enhances its stability. WTAP knockdown is shown to induce G2 accumulation, which is partially rescued by adenoviral overexpression of cyclin A2. WTAP-null mice exhibit proliferative failure with early embryonic death. WTAP is also reported to be an oncogenic protein in acute myeloid leukemia (AML) and its increased expression has been linked to chemoresistance in AML. Ref: Horiuchi, K., et al. (2006), Proc. Natl. Acad. Sci. USA 103, 17278-17283. Bansal, H., et al. (2014). Leukemia 28, 1171-1174.

This polyclonal antibody targets an internal sequence within the N-terminal half of human WTAP.

KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human WTAP.

Anti-WTAP, Cat. No. ABE555, is a highly specific rabbit polyclonal antibody that targets WTAP and has been tested in Western Blotting.

Research Category
Epigenetics & Nuclear Function

Evaluated by Western Blotting in A431.

Western Blotting Analysis: A 1:250 dilution of this antibody detected WTAP in 10 µg of A431 cell lysate.

~52 observed. 44.24/17.80 kDa (isoform 1/2 calculated). Uncharacterized bands may be observed in some lysate(s).

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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