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ABN1372

Sigma-Aldrich

Anti-Potassium Channel KCNQ5 Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
Potassium voltage-gated channel subfamily KQT member 5, Potassium channel subunit alpha KvLQT5, KQT-like 5, Voltage-gated potassium channel subunit Kv7.5, KCNQ5
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat

species reactivity (predicted by homology)

canine (based on 100% sequence homology), feline (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), giant panda (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NP_062816

UniProt accession no.

Q9NR82

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... KCNQ5(56479)

General description

KCNQ5 is subunit one of a voltage gated potassium channel expressed in the brain and other tissues. KCNQ5 forms the complete channel with KCNQ3. This channel gives rise to the "M" potassium current which is a slowly activating and deactivating current. This current plays a critical role in determining the subthreshold electrical excitability of neurons. KCNQ5 is strongly expressed in the brain but also in skeletal muscle and vascular smooth muscle cells. KCNQ5 activity is also regulated via phosphorylation by PKC and that kinase regulation may be a key to regulating excitability in neurons and muscle cells.

Specificity

Western Blotting Analysis was tested on overxpressing transfected cell lysate.

Immunogen

KLH-conjugated linear peptide corresponding to the Human Potassium Channel KCNQ5.

Application

Research Category
Neuroscience
This Anti-Potassium Channel KCNQ5 Antibody is validated for use in Immunohistochemistry and Western Blotting for the detection of Potassium Channel KCNQ5.
Western Blotting Analysis: A 0.1µg/mL dilution from a representative lot detected Potassium Channel KCNQ5 in 10 µg of transient over-expression of Potassium Channel KCNQ5 in HEK293 cell lysate.

Quality

Evaluated by Immunohistochemistry in human cerebral cortex tissue.

Imunnohistochemistry Analysis: A 1:2,000 dilution of this antibody detected Potassium Channel KCNQ5 in human cerebral cortex tissue.

Target description

~103 kDa observed. Predicted to cross react to Isoforms 1, 2, and 3. Additional bands are detected in endogenous lysates.

Linkage

Replaces: AB5599

Physical form

Antigen Affinity Purified
Purified rabbit Polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Samuel N Baldwin et al.
British journal of pharmacology, 180(2), 174-193 (2022-09-11)
Kcnq-encoded KV 7 channels (termed KV 7.1-5) regulate vascular smooth muscle cell (VSMC) contractility at rest and as targets of receptor-mediated responses. However, the current data are mostly derived from males. Considering the known effects of sex, the oestrous cycle
Heun Soh et al.
Proceedings of the National Academy of Sciences of the United States of America, 119(13), e2117640119-e2117640119 (2022-03-24)
KCNQ2 and KCNQ3 channels are associated with multiple neurodevelopmental disorders and are also therapeutic targets for neurological and neuropsychiatric diseases. For more than two decades, it has been thought that most KCNQ channels in the brain are either KCNQ2/3 or
Loss-of-function variants in the KCNQ5 gene are implicated in genetic generalized epilepsies.
Kr??ger, et al.
EBioMedicine, 84, 104244-104244 (2022)

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