Anti-RanGAP

Ran GTPase-activating protein (UniProt: Q9VIW3; also known as RanGAP, Protein segregation distorter) is encoded by the RanGAP (also known as Sd, CG9999) gene (Gene ID: 35223) in Drosophila melanogaster. Ran is a small GTPase located predominantly in the nucleus. Along with its cofactors, RanGAP and RanGEF (Ran guanine nucleotide exchange factor), Ran is required for nuclear transport, cell cycle regulation, mitotic spindle formation, and post-mitotic nuclear envelope assembly. RanGAP is a GTPase activator for the nuclear Ras-related regulatory protein Ran and converts it to the putatively inactive GDP-bound state. It has been shown that the cytoplasmic localization of RanGAP and the nuclear localization of RanGEF are important in establishing a concentration gradient of RanGTP across the nuclear envelope that is needed for proper functioning of the Ran signaling pathway. In Drosophila, Sd, a dominant neomorphic mutation, encodes a truncated form of RanGAP that lacks 234 amino acids at the C- terminus and acts as a trans-acting factor that is essential for meiotic distortion. This distortion is observed in flies that carry the RanGAP tandem duplication and express a RanGAP truncated protein. Both full length and truncated forms of RanGAP are expressed in Drosophila testis and the truncated form also displays enzymatic activity. (Ref.: Kusano A et al. (2002). Proc. Natl. Acad. Sci. USA 99(10): 6866-6870).

This rabbit polyclonal antibody detects the wild-type RanGAP as well as a truncated form of RanGAP. It targets an epitope within the first 251 amino acids from the N-terminal end.

Epitope: N-terminus

His-tagged recombinant fragment corresponding to te first 251 amino acids from the N-terminal end of Drosphila RanGAP.

Anti-RanGAP, Cat. No. ABN1674, is a highly specific rabbit polyclonal antibody that targets Ran GTPase-activating protein and has been tested in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Immunocytochemistry Analysis: A representative lot detected RanGAP in Drosophila primary spermatocytes and salivary gland cells (Kusano, A., et. al. (2001). Dev Cell. 1(3):351-61).

Immunohistochemistry Analysis: A representative lot detected RanGAP in human C9-ALS motor cortex (Zhang, K., et. al. (2015). Nature. 525(7567):56-61).

Western Blotting Analysis: A representative lot detected RanGAP in Drosophila testis (Merrill, C., et. al. (1999). Science. 283(5408):1742-5;Kusano, A., et. al. (2001). Dev Cell. 1(3):351-61.

Immunocytochemistry Analysis: A representative lot detected RanGAP in Drosophila primary spermatocytes (Kusano, A., et. al. (2002). Proc Natl Acad Sci USA. 99(10):6866-70).

Western Blotting Analysis: A representative lot detected RanGAP in purified human RanGAP1 (Zhang, K., et. al. (2015). Nature. 525(7567):56-61).

Research Category
Neuroscience

Evaluated by Western Blotting in SD-RanGAP Mad fly testis & Wild-type Fly testis.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RanGAP in SD-RanGAP Mad fly testis & Wild-type Fly testis.

66/39 kDa observed; 66.07 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Format: Unpurified

Rabbit polyclonal antiserum with 0.05% sodium azide.

Unpurified

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.