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Merck
CN

ABN34

Sigma-Aldrich

Anti-PDE11A Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Dual 3′,5′-cyclic-AMP and -GMP phosphodiesterase 11A, cAMP and cGMP phosphodiesterase 11A

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

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biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

species reactivity (predicted by homology)

rat (based on 100% sequence homology), bovine (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NP_058649

UniProt accession no.

Q9HCR9

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PDE11A(50940)

General description

The PDE11A gene encodes for a dual-specificity Phosphodiesterase (PDE), which is expressed in the adrenal cortex and is partially inhibited by tadalafil and other PDE inhibitors. PDEs are known to catalyze cAMP and cGMP to 5’-AMP and 5’GMP, respectively and thereby regulate the intracellular concentration of these nucleotides. Loss-of-function mutation of the PDE11A gene is shown to increase cAMP signaling and cAMP-responsive element binding protein (CREB) phosphorylation, two conditions which are associated with adrenocortical hyperplasia, adenomas, and Cushing syndrome.
~104 kDa observed. Uniprot states four isoforms at a molecular weight of ~105 kDa, ~78 kDa, ~66 kDa, and ~56 kDa This antibody can only recognize the ~105 kDa and the ~78 kDa isoforms.

Immunogen

Epitope: GAF domain.
KLH-conjugated linear peptide corresponding to the GAF domain of human PDE11A.

Application

Detect PDE11A using this Anti-PDE11A Antibody validated for use in WB, IH.
Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected PDE11A in normal mouse midbrain tissue.
Research Category
Neuroscience
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

Biochem/physiol Actions

This antibody recognizes GAF domain of PDE11A.

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human hippocampus tissue lysate
Evaluated by Western Blot in human hippocampus tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected PDE11A in 10 µg of human hippocampus tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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