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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IHC, WB
Citations:
-
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, human
technique(s)
immunohistochemistry: suitable, western blot: suitable
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NENF(29937)
General description
NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor.
~37 kDa observed
Immunogen
KLH-conjugated linear peptide corresponding to human NENF.
Application
Detect the NENF protein using this Anti-NENF validated for use in WB & IHC.
Immunohistochemistry Analysis: 10 µg/mL of this antibody from a representative lot detected NENF in rat kidney tissue.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neuronal & Glial Markers
Physical form
Purified rabbit polyclonal in buffer containing PBS and 0.02% sodium azide.
Antigen Affinity Purified
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human kidney tissue lysate
Human kidney tissue lysate
Evaluated by Western Blot in human kidney tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected NENF in 15 µg of human kidney tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected NENF in 15 µg of human kidney tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| ABN352 | 04053252847592 |