Anti-SPTLC1 Antibody

Serine palmitoyltransferase 1 (EC 2.3.1.50; UniProt O15269; also known as LCB 1, Long chain base biosynthesis protein 1, Serine-palmitoyl-CoA transferase 1, SPT 1, SPT1) is encoded by the SPTLC1 (also known as HSAN1, HSAN1A, LCB1) gene (Gene ID 10558) in human. Serine palmitoyltransferase (SPT) catalyzes pyridoxal 5-phosphate-/PLP-dependent condensation of L-serine with palmitoyl-CoA to generate 3-ketodihydrosphingosine, the rate-limiting step for the de novo synthesis of sphingolipids. SPT belongs to the POAS (PLP-dependent α-oxoamine synthase) family of enzymes, members of this family also include 5-amino-levulinic acid synthase, KBL (2-amino-3 ketobutyrate ligase) and AONS (8-amino-7-oxononanoate synthase). Unlike the other known POAS family members that all exist as soluble homodimers, SPT is bound to the outer membrane of the endoplasmic reticulum (ER) as a multisubunit complex composed of three distinct subunits, SPTLC1, SPTLC2 and SPTLC3. SPTLC1 is a 473-amino acid sigle-transmembrane (a.a. 16-36) protein with a short N-terminal lumenal domain (a.a. 1-15) and a large C-terminal cytoplasmic domain (a.a. 37-473). Alternative splicing generates an isoform with a truncated cytoplasmic domain (D143 switched to E143, a.a. 144-473 truncated).

Expected to react with both spliced isoforms of human Serine palmitoyltransferase 1/SPTLC1 reported by UniProt (O15269).

Epitope: Cytoplasmic domain.

Two linear peptides corresponding to sequences from the N- and a C-terminal regions of human SPTLC1 cytoplasmic domain.

Anti-SPTLC1 Antibody is an antibody against SPTLC1 for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Lipid Signaling

Western Blotting Analysis: The purified polyclonal antibody was used to detect the presence of SPTLC1 in SPTLC2 and SPTLC3 immunoprecipiates from human placenta tissue homogenate (Hornemann, T., et al. (2007). Biochem. J. 405(1):157-164).
Western Blotting Analysis: The purified polyclonal antibody was used to detect SPTLC1 in human cell lysates (HEK293, HepG2, JAR, JEG3) and human placenta tissue homogenate (Hornemann, T., et al. (2006). J. Biol. Chem. 281(49):37275-37281).

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected SPTLC1 in 10 µg of HepG2 cell lysate.

~51 kDa observed. 52.74 kDa (isoform 1) and 16.07 kDa (isoform 2) calculated.

Rabbit polyclonal antibody serum with 0.05% sodium azide.

Unpurified

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to lot specific datasheet.

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