Vacuolar protein-sorting-associated protein 25 (UniProt: Q9BRG1; also known as hVps25, Dermal papilla-derived protein 9, ELL-associated protein of 20 kDa, ESCRT-II complex subunit VPS25) is encoded by the VPS25 (also known as DERP9, EAP20) gene (Gene ID: 84313) in human. VPS25 is a component of the ESCRT-II complex (endosomal sorting complex required for transport II) that is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. VPS25 is diffusely distributed throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on co-expression with CHMP6. It is expressed at the mRNA level in kidney, liver, pancreas, and placenta. Lower levels of expression have been reported in heart, skeletal muscle, brain and lung.

~20 kDa observed; 20.75 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Epitope: unknown

GST-tagged full length human recombinant VPS25 protein.

Anti-VPS25, Cat. No. ABS1676, is a rabbit polyclonal antibody that targets Vacuolar protein-sorting-associated protein 25 and has been tested for use in Immunofluorescence and Western Blotting.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected VPS25 in Cos-7 cells (Langelier, C., et. al. (2006). J Virol. 80(19):9465-80).

Western Blotting Analysis: A 1:500 & 1:1,000 dilution from a representative lot detected VPS25 in lysate of transfected HEK293T cells (Sandrin, V., et. al. (2013). Retrovirology. 10:104; Langelier, C., et. al. (2006). J Virol. 80(19):9465-80).

Research Category
Signaling

This polyclonal antibody specifically detects Vacuolar protein-sorting-associated protein 25 (VPS25) in human and monkey cells.

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected VPS25 in 10 µg of HEK293 cell lysate.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.