Anti-CDK11

Cyclin-dependent kinase 11A (UniProt: Q9UQ88; also known as EC:2.7.11.22, Cell division cycle 2-like protein kinase 2, Cell division protein kinase 11A, Galactosyltransferase-associated protein kinase p58/GTA, PITSLRE serine/threonine-protein kinase CDC2L2) is encoded by the CDK11A (also known as CDC2L2, CDC2L3, PITSLREB) gene (Gene ID: 728642) in human. CDK11 is a ubiquitously expressed protein that plays multiple roles in cell cycle progression, cytokinesis, and apoptosis. Eight different isoforms of CDK11 have been described that are produced by alternative splicing and alternative initiation. The p110 isoform (canonical) is suggested to be involved in pre-mRNA splicing, potentially by phosphorylating the splicing protein SFRS7. During apoptosis, it is induced by Fas or tumor necrosis factor. The p110 isoform is cleaved by caspases to produce p110C form that contains the C-terminal kinase domain and binds to the serine/threonine kinase PAK1. CDK11 is reported to associate with SF3B1 and phosphorylates threonine residues at its N terminus during spliceosome activation. This phosphorylation event is shown to be essential for the association between SF3B1 and U5 and U6 snRNAs in the activated spliceosome, termed the Bact complex. CDK11 is overexpressed in several tumor types and is a predictor of poor clinical outcome. (Ref.: Blazek, D. (2023). Clin. Transl Med. 13(3); e1201; Hluchý, M., et al. (2022). Nature. 609(7928); 829-834).

His-tagged recombinant fragment corresponding to the first 226 amino acids from the N-terminal region of human Cyclin-dependent kinase 11A (CDK11A).

Quality Control Testing

Evaluated by Western Blotting in HCT116 Cell Lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CDK11 in HCT116 cell lysate.

Tested Applications

Western Blotting Analysis: A representative lot detected Cyclin-dependent kinase 11 in Western Blotting applications (Hluchy, M., et. al. (2022). Nature. 609(7928):829-834).

ChIP-seq Anaylysis: A representative lot detected Cyclin-dependent kinase 11 in ChIP-seq applications (Hluchy, M., et. al. (2022). Nature. 609(7928):829-834).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

This rabbit polyclonal antibody detects Cyclin-dependent kinase 11A (CDK11) and targets an epitope within the N-terminal region.

Rabbit polyclonal antibody in serum without azide.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.