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Merck
CN

ABS30

Anti-Sulfenic Acid Modified Cysteine (2-Thiodimedone-Specific Ig) Antibody

serum, from rabbit

Synonym(s):

Sulfenic Acid Modified Cysteine (2-Thiodimedone-Specific Ig)

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human, mouse, rat
Application:
WB
Citations:
9
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biological source

rabbit

Quality Segment

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse, rat

species reactivity (predicted by homology)

all

technique(s)

western blot: suitable

isotype

IgG

shipped in

wet ice

target post-translational modification

unmodified

General description

Protein sulfenic acid formation is a reversible post-translational modification that may be used to monitor protein oxidation on reactive cysteines within target proteins. This can be detected with protein sulfenic acid derivatised with dimedone.
Pan antibody smear is expected.

Immunogen

Linear peptide corresponding to sulfenic acid modified proteins.

Application

Anti-Sulfenic Acid Modified Cysteine (2-Thiodimedone-Specific Ig) Antibody detects level of Sulfenic Acid Modified Cysteine & has been published & validated for use in WB.
Immunofluorescence Analysis: A previous lot was used by an independent laboratory in IF. (Seo, YH, et al. (2009). PNAS. 106(38): 16163-16168.)

Biochem/physiol Actions

This anitbody recognizes sulfenic acid modified proteins. Pan modification against all species.

Analysis Note

Evaluated by Western Blot in rat ventricular myocyte lysate.

Western Blot Analysis: 01:1,000 dilution of this antibody detected sulfenic acid modified proteins on 10 µg of rat ventricular myocyte lysate.


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Storage Class

10 - Combustible liquids

wgk

WGK 1



Certificates of Analysis (COA)

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Global Trade Item Number

SKUGTIN
ABS3004053252517587