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Merck
CN

ABS441

Anti-Fat Free Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Fat Free homolog, Vacuolar protein sorting-associated protein 51 homolog

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
WB
Species reactivity:
mouse
Citations:
Technique(s):
western blot: suitable
Uniprot accession no.:
Q9UID3

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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

species reactivity (predicted by homology)

human (immunogen homology)

technique(s)

western blot: suitable

UniProt accession no.

Q9UID3

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... ANG(283)

Analysis Note

Control
Mouse brain tissue lysate
Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Fat Free in 15 µg of mouse brain tissue lysate.

Application

Anti-Fat Free Antibody is a highly specific rabbit polyclonal antibody, that targets Fat free & has been tested in western blotting.
Research Category
Signaling
Research Sub Category
Lipid Metabolism & Weight Regulation

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Protein fat-free homolog (Fat Free), also known as Vacuolar protein sorting-associated protein 51 homolog and Another new gene 2 protein (ANG2). Fat Free is a component of the Golgi-associated retrograde protein (GARP) complex which mediates the tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN). The GARP complex maintains protein retrieval from endosomes to the TGN, acid hydrolase sorting, lysosome function, endosomal cholesterol traffic and autophagy. Fat Free participates in acid hydrolase receptor transport, likely by promoting tethering and fusion of endosome-derived carriers to the TGN.
~80 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~73 kDa and ~86 kDa

Immunogen

KLH-conjugated linear peptide corresponding to human Fat Free.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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