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Merck
CN

ABS984

Anti-Intersectin-1 Antibody

serum, from rabbit

Synonym(s):

ITSN-1, ITSN1, Intersectin-1, Intersectin1, ITSN 1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunocytochemistry
immunoprecipitation (IP)
western blot
Species reactivity:
mouse, human, rat
Citations:
1
Technique(s):
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Uniprot accession no.:
O42287

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Product Name

Anti-Intersectin-1 Antibody, serum, from rabbit

biological source

rabbit

conjugate

unconjugated

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human, rat

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NP_001080955

UniProt accession no.

O42287

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... ITSN1(6453)

Analysis Note

Evaluated by Western Blotting on rat brain cytosol tissue lysate

Western Blotting Analysis: A 1:1000 dilution of this antibody detected Intersectin-1 protein in 10 µg of rat brain cytosol.

Application

Anti-Intersectin-1 is an antibody against Intersectin-1 for use in WB, ICC, IP.
Immunocytochemistry Analysis: A representative lot of this antibody detected Intersectin-1 protein in HEK293T cells (Martin, N., et al. Mol Pharmacol 70:1643–1653, 2006)
Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipiated Intersectin-1 (short and long isoforms) from mouse brain lysate (Martin, N., et al. Mol Pharmacol 70:1643–1653, 2006)

General description

Intersectin-1 is a scaffolding protein facilitating clathrin-mediated endocytosis, vesicle trafficking and mitogen-activated protein kinase (MAPK) signaling pathways. Functional association studies of the protein have suggested ITSN1 has several differing roles in various processes such as RAS activation and stimulation of Cdc42 in order to accelerate actin assembly. Alternative splicing produces 4 isoforms. The major isoforms are ITSN1-L and ITSN1-S. Expression for ITSN1 is ubiquitous in adult/fetal tissue, except isoform 1 which is mostly found in the brain. The most notable expression is found in muscle, spleen, skeletal muscle, testis, heart, ovary and all fetal tissue. Additionly, the neurons of young Down Syndrome and pre-Alzheimer’s disease brains are predisposed for enlarged early endosomes which may contribute to these pathological processes.
~150 and ~200 kDa observed. Previous publication indicated the 200 kDa to be the long isoform and 150 kDa to be the short isoform (Okur, M., et al. Mol. Cell. Biol. 2014, 34(2):271). Uncharacterized bands may be detected at high antibody concentrations or longer exposures.

Immunogen

Epitope: SH3 domain of Xenopus Intersectin-1 protein
GST-tagged recombinant protein corresponding to the SH3 domain of Xenopus Intersectin-1.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class

10 - Combustible liquids

wgk

WGK 1

Certificates of Analysis (COA)

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