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Merck
CN

ABS986

Anti-PCTP Antibody

serum, from rabbit

Synonym(s):

Phosphatidylcholine transfer protein, PC-TP, START domain-containing protein 2, StARD2, StAR-related lipid transfer protein 2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IP, WB
Citations:
2

Key Documents

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biological source

rabbit

conjugate

unconjugated

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

NCBI accession no.

NP_067036

UniProt accession no.

Q9UKL6

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... PCTP(58488)

General description

Phosphatidylcholine transfer protein (PCTP), also known as PC-TP, START domain-containing protein 2, StARD2, StAR-related lipid transfer protein 2, and encoded by the gene symbol PCTP/ STARD2, belongs to the steroidogenic acute regulatory protein related transfer (START) domain superfamily of hydrophobic ligand-binding proteins. PCTP has been shown to play an important role in the transfer of phosphatidylcholine between membranes. The crystal structures of human PC-TP in complex with dilinoleoyl-PtdCho or palmitoyl-linoleoyl-PtdCho suggests that a single well-ordered PtdCho molecule occupies a centrally located tunnel. These structures have provided a basis for rationalizing the specificity of PC-TP for PtdCho and may identify common features used by START proteins to bind their hydrophobic ligands. Additionally, PCTP has been demonstrated to interact with ACOT13/THEM2. PCTP is located in the cytoplasm with highest expression in liver, placenta, testis, kidney and heart. Low levels of PCTP are expressed in brain and lung.
~33 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human PCTP.

Application

Anti-PCTP is an antibody against PCTP for use in WB, ICC, IP.
Western Blotting Analysis: A representative lot detected PCTP in cytosolic fractions isolated from the liver of a control subject, a patient with intrahepatic brown pigment stones, and a patient with obstructive cholestasis (Shoda, J., et al. (2001). Hepatology. 33(5):1194-1205).
Western Blotting Analysis: A representative lot detected PCTP in siRNA-transfected, serum-starved HEK 293E cells, Pctp+/+ liver lysates, Tsc2−/− MEFs, Liver homogenates from chow diet–fed Pctp+/+ (n = 4) and Pctp−/− (n = 4) mice (Ersoy, B.A., et al. (2013). Science Signaling. 286(6):1-11).
Immunocytochemistry Analysis: A representative lot detected PCTP in HEK293T cells (Kanno, K., et al. (2007). J. Biol. Chem. 282:30728-30736).
Immunoprecipitation Analysis: A representative lot immunoprecipitated PCTP in Pctp+/+ liver lysates (Ersoy, B.A., et al. (2013). Science Signaling. 286(6):1-11).

Analysis Note

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PCTP in 5 µg of HepG2 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class

10 - Combustible liquids

wgk

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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