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Merck
CN

ACR5000NT

Ni-NTA Reagent Kit

suitable for protein purification

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About This Item

UNSPSC Code:
41104916
NACRES:
NA.56
eCl@ss:
36100101
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product line

Amicon®

technique(s)

protein purification: suitable (His-tagged proteins)

shipped in

wet ice

General description

For use with the Amicon Pro Affinity Conc: 3 ml HisBind Ni-NTA resin, 25ml Bind Buffer (4X), 25 ml Wash buffer (4X), 5ml elution buffer (4X)
Ni-NTA Reagent Kit for His-tagged protein purification.

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany


signalword

Danger

Hazard Classifications

Eye Dam. 1 - Flam. Liq. 3 - Repr. 1B - Skin Corr. 1C

Storage Class

3 - Flammable liquids

flash_point_f

95.9 °F

flash_point_c

35.5 °C

wgk

WGK 2



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Protein purification is simpler with Amicon Pro. The device delivers consistent sample preparation for more reliable recovery, uncompromised purity and easier data generation.

Amicon Pro 让蛋白纯化更简单。此系统可提供一致的样品制备,可实现更可靠的蛋白回收率、同时保证纯度、简化数据生成。

Related Content

Two attributes of the Amicon® Pro device make it a convenient device for preparing pure, labeled antibody. First, the device enables highly efficient buffer exchange via diafiltration with simultaneous sample concentration in a single 15 minute spin. Second, the entire workflow can be performed within a single device, reducing the potential for sample loss. In this report, we describe the successful use of the Amicon® Pro device for small–scale biotinylation of a target antibody. The biotinylated antibody was used for detection of protein immunoprecipitated from cell lysate.

When you start with consistent yields of active, native-folded protein, you’re giving your experiment the best chance to succeed. If your current protein preps involve juggling columns, dialyzers and multiple transfer steps, you could be introducing variability into your data. For your next protein preparation, choose the simple, gentle method that tackles even the most labile and poorly expressed proteins—the Amicon® Pro purification system. When your proteins behave, your research will flourish.

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