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Merck
CN

ACR5000PG

Protein G Reagent Kit

for antibody purification

Synonym(s):

Protein purification kit

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About This Item

UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24
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General description

Protein G is a bacterial cell wall protein produced by G Streptococci that binds to immunoglobulin (IgG). It is a single polypeptide chain with minimal carbohydrate modifications. Native protein G consists of two IgG binding domains and binding sites for cell surface and albumin. The Amicon® Pro Affinity Concentration Kit - Protein G is developed for maximal binding and elution of Ig species.

Application

Amicon® Pro Affinity Concentration Kit - Protein G is ideal for antibody purification.

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

FlameCorrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Flam. Liq. 2 - Met. Corr. 1

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Protein purification is simpler with Amicon Pro. The device delivers consistent sample preparation for more reliable recovery, uncompromised purity and easier data generation.

Amicon Pro 让蛋白纯化更简单。此系统可提供一致的样品制备,可实现更可靠的蛋白回收率、同时保证纯度、简化数据生成。

Related Content

When you start with consistent yields of active, native-folded protein, you’re giving your experiment the best chance to succeed. If your current protein preps involve juggling columns, dialyzers and multiple transfer steps, you could be introducing variability into your data. For your next protein preparation, choose the simple, gentle method that tackles even the most labile and poorly expressed proteins—the Amicon® Pro purification system. When your proteins behave, your research will flourish.

Two attributes of the Amicon® Pro device make it a convenient device for preparing pure, labeled antibody. First, the device enables highly efficient buffer exchange via diafiltration with simultaneous sample concentration in a single 15 minute spin. Second, the entire workflow can be performed within a single device, reducing the potential for sample loss. In this report, we describe the successful use of the Amicon® Pro device for small–scale biotinylation of a target antibody. The biotinylated antibody was used for detection of protein immunoprecipitated from cell lysate.

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