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Merck
CN

AP308F

Sigma-Aldrich

Goat Anti-Mouse IgG Antibody, (H+L) FITC conjugate

1.15 mg/mL, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

Key Documents

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biological source

goat

Quality Level

100

conjugate

FITC conjugate

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

concentration

1.15 mg/mL

technique(s)

immunofluorescence: suitable

shipped in

wet ice

target post-translational modification

unmodified

Related Categories

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
The reagent is an affinity purified antibody from goat. The purified antibody is conjugated to fluorescein and stabilized in buffer.

Application

Detect Mouse IgG using this Goat anti-Mouse IgG Antibody, (H+L) FITC conjugate validated for use in IF.
F/P RATIO: 4-6

Immunohistochemistry: 1:200-1:500 (1-4)

Immunocytochemistry: 1:200-1:500 (1-4)

Flow Cytometry: 1 μg per 1x 10E6 cells (1,2,4) FITC absorption emission is 488-492nm, its emission peak is 520nm.

Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Biochem/physiol Actions

Specific for mouse IgG, heavy and light chain. The cross-reactivities of anti-mouse IgG antibody are tested in an ELISA. Minimum cross-reactivity to human IgG.

Physical form

ImmunoAffinity Purified
Lyophilized. Buffer = 0.02M Phosphate Buffer, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA and 0.1% Sodium azide. Reconstitute with 1 mL of sterile distilled water.

Preparation Note

Maintain lyophilized product at 2–8°C for up to 12 months after date of receipt. After reconstitution the product is stable for several weeks at 2–8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles. Protect from light.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Eda Becer et al.
Turkish journal of pharmaceutical sciences, 17(3), 265-270 (2020-07-09)
Mesenchymal stem cells are self-renewing stem cells. The human foreskin has potential to be used as a source of stem cells. The aim of the study was to obtain spheroid formation of human foreskin cells (hnFSSCs) isolated from newborn human
Effect of selegiline on neural stem cells differentiation: a possible role for neurotrophic factors.
Hassanzadeh, K; Nikzaban, M; Moloudi, MR; Izadpanah, E
Iranian Journal of Basic Medical Sciences null
Chorioallantoic placentation in Galea spixii (Rodentia, Caviomorpha, Caviidae).
Oliveira, MF; Mess, A; Ambrosio, CE; Dantas, CA; Favaron, PO; Miglino, MA
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Andrea M Mess et al.
Reproductive biology and endocrinology : RB&E, 10, 102-102 (2012-12-04)
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