IgG is one of the most abundant protein in human serum. IgG is important for our defence against microorganisms and the molecules are produced by B-lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Epitope: IgG γ chain

Prepared from purified mouse IgG γ chain. Pooled antisera from goats hyperimmunized with mouse IgG paraproteins.

Goat anti-Mouse IgG γ chain Antibody, TRITC conjugate, Species Adsorbed detects level of Mouse IgG γ chain & has been published & validated for use in ELISA & IF.

Immunofluorescence: ≤1 μg/10⁶ cells.
ELISA

Optimal working dilutions must be determined by the end user.

Research Category
Secondary & Control Antibodies

Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Fragment Specific Secondary Antibodies

Reacts with heavy chain of mouse IgG1, IgG2a, IgG2b and IgG3. Absorbed for mouse IgM and IgA, pooled human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.

The antibody reacts with the heavy chain of mouse IgG1, IgG2a, IgG2b and IgG3 as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.

Affinity Chromatography

Purified by affinity chromatography on pooled mouse IgG covalently linked to agarose. Liquid in 1.0 mL PBS/NaN₃.

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. do not freeze.

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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.