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CA1023

Anti-Hsp90α Mouse mAb (EMD-17D7)

Name: Anti-Hsp90α Mouse mAb (EMD-17D7)
Brand: MM

liquid, clone EMD-17D7, Calbiochem^®

Synonym(s):

Anti-Heat Shock Protein 90α

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Clone:

EMD-17D7, monoclonal

Species reactivity:

human

Application:

—

Citations:

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Properties

biological source

mouse

Quality Segment

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

EMD-17D7, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(ELISA
Immunoblotting (1 µg/mL)
Immunoprecipitation (5 µg/0.7 mg protein))

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Protein G PLUS/protein A purified mouse monoclonal antibody. Recognizes the ~90 kDa Hsp90α protein.

Recognizes the ~90 kDa Hsp90α protein in HeLa cells. Does not recognize Hsp90β.

This Anti-Hsp90α Mouse mAb (EMD-17D7) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of Hsp90α.

Immunogen

Hsp90α, His•Tag, Human, Recombinant (Cat. No. 385901)

Human

Application

ELISA (see comments)
Immunoblotting (1 g/ml)
Immunoprecipitation (5 g/0.7 mg protein)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
H460 and HeLa cells

Other Notes

Detects native and recombinant Hsp90α. Does not recognize Hsp90β. This antibody will also work for ELISA, but concentration is assay dependent. Antibody should be titrated for optimal results in individual systems.

Scheibel, T., et al. 1999. Proc. Natl. Acad. Sci. USA96, 1297.
Nemoto, T., et al. 1997 J. Biol. Chem.272, 26179.
Nemoto, T., et al. 1998. Biochem. J.330, 989.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c